Stilbene Molecular Probes as Potential Materials for Bioengineering: Real Time Analysis of Antioxidants and Nitric Oxide, Immunoassay in Solution and Biomembranes Fluidity

Abstract:

A series of fluorescent methods of analysis and investigation of system based on the use of stilbenes and potentially important in biochemistry, biophysics, biotechnology, and biomedicine were proposed and developed. In these methods, two new types of stilbene molecular probes have been used: (i) fluorescent photochrome molecules and (ii) super molecules containing fluorescent and fluorescent quenching segments. These methods utilize the following photochemical and photophysical phenomena: the fluorescence and phosphorescence quenching, photochrome photoisomerization, and triplet–triplet and singlet–singlet energy transfer. The fluorescence properties of the new probes were intensively exploited as the basis of several methodologies that include a real-time analysis of nitric oxide, immunoassay in solution, investigation of molecular dynamics of biomembranes in a wide range characteristic times, and characterization of sensors for antibodies. These techniques may be adapted to fibro-optic sensoring.

• Periodical: Advanced Materials Research (Volume 699)
• Pages: 718-723
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.699.718
• Online since: May 2013
• Authors: Gertz I. Likhtenshtein
• Keywords: Aspartic Acids, Biomembrane Fluidity, Fluorescent-Photochrome Method, Immunoassay, Nitric Oxide, Singlet-Singlet Energy Transfer, Stilbenes, Triplet-Triplet Energy Transfer

References

[1] G.I. Likhtenshtein, Stilbenes: Application in Chemistry, Life Science and Material Science. WILEY-VCH, Weinhem, 2009.

[2] G.I. Likhtenshtein Gertz I. (2012) Stilbenes: Chemistry and application. In Kirk-Othmer Encyclopedia of Chemical Technology. Published Online: 13 JAN 2012.

DOI: 10.1002/0471238961.stillkh.a01

[3] G.I. Likhtenshtein, D.V. Khudjakov, and V.R. Fogel,. J. Biochim. Biophys Methods, vol. 25, pp.219-229, (1992)

[4] V. Mekler, V., and G.I. Likhtenshtein, G.I. Study of dynamic contacts of macromolecules according to kinetics of photoisomrization of fluorescent label Biofizika, Vol. 31, pp.571-586, 1986.

[5] G.I. Likhtenshtein, R. Bishara, V. Papper., B. Uzan, I. Fishov I., D. Gill , A.H. Parola,. J. Biochem. Biophys. Methods, Vol. 33, pp.117-133, 1996.

DOI: 10.1016/s0165-022x(96)00022-x

[6] G.I. Likhtenshtein, Spin and fluorescence immunoassay in solution. In: Immunology Methods Manual, Levkovits and Nezlin, R. (eds.) Pergamon Press, London, pp.540-550, 1996.

DOI: 10.1016/b978-012442710-5.50060-5

[7] V. Papper V., G.I. Likhtenshtein., N. Medvedeva, and D.V. Khoudyakov, , J. Photochem. Photobiol. A: Chem., Vol. 122, pp.79-85, 1999.

[8] Papper V., Medvedeva N., Fishov I., and G.I. Likhtenshtei,. J. Appl. Biotech. Biophys, vol.. 88, pp.1-17, 2000.

[9] V. Papper, and G.I. Likhtenshtein. J. Photochem. Photobiol. A: Chem, vol. .140, pp.39-52, 2001.

[10] A. Ahluwalia , V. Papper ., O. Chen ., G.I. Likhtenshtein G.I., and D. De Rossi . A fluorescence method for characterization of order and orientation of antibodies immobilized on a silica plate, Analytical Biochemistry, Vol. 305, pp.121-134, 2002.

DOI: 10.1006/abio.2002.5601

[11] P. Parkhomyuk-Ben Arye, N. Strashnikova, N., and G.I. Likhtenshtein, J. Biochem Biophys. Methods, Vol. 51, pp.1-15, 2002.

[12] O. Chen O., R. Glaser , and G.I. Likhtenshtein. Biophys. Chem., Vol. 103, pp.139-156, 2003.

[13] N. Medvedeva , V. Papper , and G.I. Likhtenshten, Phys. Chem. Chem. Phys, Vol. 7, p.3368 – 3374, 2005.

[14] O. Chen, R. Glaser , G.I. Likhtenshtein, The novel FluoroChrome ImmunoAssay — (FCIA), J. Biochem. Biophys. Methods, Vol. 70, pp.1073-1079. 2008.

DOI: 10.1016/j.jbbm.2007.07.007

[15] O. Chen, N. Uzlaner, Z. Priel, and G.I. Likhtenshtein, Journal of Biochemical and Biophysical Methods, Vol.70, pp.1006-1013, 2008.

DOI: 10.1016/j.jbbm.2007.09.005

[16] G.I. Likhtenshtein, Applied biochemistry and biotechnology, Vol. 152, pp.135-155, 2009.