Kinetics of Ferrous Iron Oxidation by Leptospirillum Ferriphilum at Moderate to High Total Iron Concentrations
The effects of temperature, pH and iron concentration on the kinetics of ferrous iron biooxidation by a free suspended culture of Leptospirillum ferriphilum were studied in shake flasks and a circulating bed bioreactor at moderate to high total iron concentration. The kinetic study showed that there are two distinct modes of iron biooxidation: growth associated and non-growth associated, depending on the pH of the medium. There were also distinctive maxima of the effect of temperature and pH on the rate of biooxidation. A kinetic model of the process was proposed, based on an electrochemical-enzymatic model. The proposed model indicates that at moderate to high concentrations (above ~12 g/L), the total iron concentration becomes the single most prominent inhibiting factor.
Edgardo R. Donati, Marisa R. Viera, Eduardo L. Tavani, María A. Giaveno, Teresa L. Lavalle, Patricia A. Chiacchiarini
K. Penev and D. Karamanev, "Kinetics of Ferrous Iron Oxidation by Leptospirillum Ferriphilum at Moderate to High Total Iron Concentrations", Advanced Materials Research, Vols. 71-73, pp. 255-258, 2009