Mass Spectrometry Analysis of Differential Protein Spots from Intestinal Side Population Cells and Epithelial Cells in Newborn Mice

Yong Feng Zhang; Jin Yang; Yong Gang Tan; Man Yin Chen; Min Yang

doi:10.4028/www.scientific.net/AMR.749.232

Paper Titles

Therapeutic Efficacy of Calcium Hydroxide as an Intra Canal Dressing on Postoperative Reaction of Patients with Residual Pulpitis
p.211

Effects of Free Radical Mediator NF-κB Expression Change on Brain in Aging Mice after Various Load Aerobic Training
p.215

Program Simulation of Cell Division and Differentiation Based on P-Code
p.221

Optimization of Fertilizer Application for Dioscorea zingiberensis C. H. Wrights
p.228

Mass Spectrometry Analysis of Differential Protein Spots from Intestinal Side Population Cells and Epithelial Cells in Newborn Mice
p.232

Isolation and Identification of Halomonas sp. ZSCW-10: A Moderately Halophilic Bacteria Strain with Cellulase Activity
p.236

Chloroplast TrnL-F Sequences Analysis of Pulsatilla koreana Nakai. and Potentilla chinesis Ser.
p.242

ITS Sequences Analysis of Pulsatilla koreana Nakai. and Potentilla chinesis Ser.
p.246

Fetal Electrocardiogram Extraction Based on Modified Robust Independent Component Analysis
p.250

HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 749Mass Spectrometry Analysis of Differential Protein...

Mass Spectrometry Analysis of Differential Protein Spots from Intestinal Side Population Cells and Epithelial Cells in Newborn Mice

Abstract:

objective: To screen differential proteins or protein groups with stem cell characteristics.methods: Two-dimensional gel electrophoresis was used to separate total proteins from intestinal side population cells and intestinal epithelial cells of neonatal mice. Proteins which were differentially expressed more than 1.5 times were selected for mass spectrometry analysis as well as database retrieval and identification. results: The two-dimensional electrophoresis patterns of intestinal side population cells and intestinal epithelial cells were successfully established to screen 39 differential protein spots. Thirty-two kinds of proteins were identified using the mass spectrometry analysis and database retrieval. conclusion: There were differences between the protein expression profiles of mouse intestinal epithelial cells and stem cells. The identified 32 kinds of proteins may become potential and valuable molecular markers for identification of intestinal stem cells.

You might also be interested in these eBooks

View Preview

Info:

Periodical:

Advanced Materials Research (Volume 749)

Pages:

232-235

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.749.232

Citation:

Cite this paper

Online since:

August 2013

Authors:

Yong Feng Zhang, Jin Yang, Yong Gang Tan, Man Yin Chen, Min Yang

Keywords:

Intestinal Epithelial Cells, Intestinal Side Population Cells, Mice, Proteomics, Two-Dimensional Electrophoresis

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

Citation:

References

[1] Zhang YF, Yang J, Wu ZZ, Jia XQ, Li M, Li YH. In vitro isolation of intestinal side population cells from newborn mice. Journal of Clinical Rehabilitative Tissue Engineering Research, 2010; 14(27): 5115-5118.

Google Scholar

[2] Wang L, Duan XL. In vitro primary culture of rat intestinal epithelial cells. Junshi Yixue Kexueyuan Yuankan, 2004, 28(1): 61-63.

Google Scholar

[3] Zhai RL, Wang GB, Cai KL, Tian Y, Xu F. Isolation, culture and identification of primary colonic epithelial cells from normal suckling rats. Zhonghua Zhongliu Fangzhi Zazhi, 2008, 15(18): 1365-1367.

Google Scholar

[4] Fu Y, Liu ZS, Jiang CQ. Research advance of markers for intestines stem cells. Linchuang Waike Zazhi, 2010, 18(1): 55-57.

Google Scholar

[5] Ye NS, Luo GA, Wang YM. Advances of Proteomics in Stem Cells Research. Shengwu Jishu Tongbao, 2006, (3): 1-4.

Google Scholar

[6] Luo Y, Yang YS. Application progress of proteomics in digestive system diseases. ZhongguoLinchuang Zonghe, 2007, 23(2): 187-190.

Google Scholar