Study on Nutrient Removal Performance and Biological Community Structure in Hybrid Biofilm Reactor

Abstract:

To investigate nutrient removal and biological community structure in wastewater treatment system under different ratio of C/N/P, hybrid biofilm reactor (HBR) and sequencing batch reactor (SBR) in lab scale were set up. Results showed that both HBR and SBR achieved the best removal efficiency of nitrogen and phosphorus when C/N/P=100/6.25/1. With the rise of C/N/P from 50/6.25/1 to 100/6.25/1, the removal efficiencies of NH₃-N were greater than 93% in three phases of both reactors; the efficiencies of TN were increased from 65% to 79% in SBR while these values at HBR were from 73% to 86%; the efficiencies of TP were increased from 42% to 93% in SBR while these values at HBR were from 32% to 93%. Microbial community structure response to different C/N/P was determined by PCR-DGGE technology. Similarity coefficient was lowest and denitrifying phosphate accumulating bacteria became dominant bacteria in SBR when C/N/P was 100/6.25/1. There were many functional bacteria in HBR, such as bacteria for removing nutrients (Uncultured BacteroidetesFJ828150), bacteria for removing organic (Uncultured Saprospiraceae) bacteria for removing nitrogen (Ferribacterium sp.), and nitrifying bacteria (Uncultured Nitrospira sp.) which became dominant bacteria. Because of microbial diversity index of biofilm was up to 3.3, similarity coefficient was as low as 43.4%.

• Periodical: Advanced Materials Research (Volume 777)
• Pages: 213-220
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.777.213
• Online since: September 2013
• Authors: Lu Zhou, Ge Li, Hang Sik Shin, Li Li Jiang, Guo Feng Fang, Li Zhen Xing, Tao Li
• Keywords: Hybrid Biofilm Reactor, Microbial Community Structure, PCR-DGGE, Removal of Nitrogen, Removal of Phosphorus, Wastewater Treatment

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