Detection and Analysis of Intracellular Polymers Stored by Polyphosphate-Accumulating Microorganisms

Abstract:

Both qualitative and quantitative analysis of intracellular polymers in polyphosphate accumulating organisms (PAOs) was discussed in this paper. Results were indicated that black-blue particles of poly-β-hydroxyalkanoate (PHBs) were observed clearly by Sudan black staining. DAPI staining and the Neisser new staining for polyphosphate (poly-P) were more proper than other staining methods. PHB content reached 198.3 mg/g MLSS detected by GC. After digested by HClO₄, the poly-P content of sludge was determined to be 138.4 mg/g MLSS.

• Periodical: Advanced Materials Research (Volume 777)
• Pages: 314-317
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.777.314
• Online since: September 2013
• Authors: Yan Hui Ge, Lin Zhao, Yan Zhou
• Keywords: Polyphosphate (Poly-P), Polyphosphate Accumulating Organisms (PAOs), Poly-β-Hydroxyalkanoate (PHB), Staining

References

[1] Mamoru Kawaharasaki, Hideo Tanaka, Takahiro Kanagawa, Kazunori Nakamura. In situ identi-fication of polyphosphate-accumulating bacteria in activated sludge by dual staining with rRNA-targeted oligonucleotide probes and 4', 6-diamidino-2-phenylindol (DAPI) at a polyphosphate-probing concentration [J]. Water Research, 1999, 33: 257-265.

DOI: 10.1016/s0043-1354(98)00183-3

[2] Yinguang Chen, Yan Liu, Qi Zhou, Guowei Gu. Enhanced phosphorus biological removal from wastewater-effect of microorganism acclimatization with different ratios of short-chain fatty acids mixture [J]. Biochemical Engineering Journal, 2005, 27: 24-32.

DOI: 10.1016/j.bej.2005.06.003

[3] Serafim L S, Lemos P C, Levantesi C, et al. Methods for detection and visuali- zation of intracellular polymers stored by polyphosphate-accumulating microorganisms [J]. Journal of Microbiological Methods, 2002, 51(1): 1-18.

DOI: 10.1016/s0167-7012(02)00056-8

[4] Pijuan M, Saunders A M, Guisasola A, Baeza J A, Casas C, Blackall L L. Enhanced biological phosphorus removal in a sequencing batch reactor using propionate as the sole carbon source [J]. Biotechnology and bioengineering, 2004, 85(1): 56-67.

DOI: 10.1002/bit.10813

[5] Environmental Protection Agency. GB11893-89 Determination of total phosphorus Molybdate spectrophotometry [S]. Beijing: China Environmental Science Press, (1989).

[6] Bartholomew J W. Stains for microorganisms in smears. In: Clark G (Ed. ), Staining Procedures, 4th edn [M]. Baltimore, London: Williams and Wilkins. 1981: 375-440.

[7] Ren S Y, Xiao T. Staining methods for intracellular polymers deposited by polyphosphate-accumulating microorganisms [J]. Marine Sciences, 2005, 29(1): 59-63.

[8] Streichan M, Golecki J R, Schon G. Polyphosphate-accumulating bacteria from sewage plant with different processes of biological phosphorus removal [J]. FEMS Microbiology Ecology, 1990, 73(2): 113-124.

DOI: 10.1111/j.1574-6968.1990.tb03931.x