Experimental Study on Oil-Degradation Microbial Consortium from Oil-Contaminated Soil by Different Enrichment and Domestication

Abstract:

In order to increase the effectiveness of remediation on oil-contaminated soil, culture techniques and molecular biological methods were adopted to analyze community structure enriched and domesticated by different ways. The results showed that the bacterial concentration cultured in a medium of inorganic salts and oil was 1.30×10¹²cfu/ml, while the bacterial concentration cultured in a medium of LB and oil was 3.05×10⁸cfu/ml; Within the perspective of structure, the microbial community cultured in a medium of inorganic salts and oil contained 11 kinds of bacterial, Meanwhile, the microbial community cultured in a medium of LB and oil was composed of 4 kinds of bacteria. Based on the results of this study, it can be inferred that the cultured medium has a significant influence on the composition of microbial communities. When domesticated oil-contaminated soil was cultured in a medium of inorganic salts and oil, the microbial concentrations and diversities were relatively higher than those cultured in LB and oil.

• Periodical: Advanced Materials Research (Volumes 779-780)
• Pages: 1304-1308
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.779-780.1304
• Online since: September 2013
• Authors: Zhi Rong Wang, Yan Ni Li, Xiao Yan Zhu, Wei Li Wang, Jing Lan Wang
• Keywords: Domestication, Enrichment, Microbial Consortium, Oil, Soil

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