Construction of Genome Library for the Toxic Gene of Alexandium minutum

Li Wang; Jia Yuan Li; Qi Xu; Qing Ping Zhong; Zhen Lin Liao

doi:10.4028/www.scientific.net/AMR.779-780.235

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 779-780Construction of Genome Library for the Toxic Gene...

Construction of Genome Library for the Toxic Gene of Alexandium minutum

Abstract:

Genome library of toxic Alexandium minutum were constructed. A 1014bp DNA reconstruction fragment was obtained and PCR method testified that this fragment had strong amplification signal in toxic strains while no signal in non-toxic strains. The external sequence of the DNA fragment was analyzed by inverse PCR method, and a 240bp nucleotide sequence which translated into proteins aminophenol was Methionine Aminopeptidase (MAP). The sequence of aminophenol was BLAST in NCBI, and found that it had 97% similarity with Alexandium fundyense. This sequence was exact uniform with the reported sequence in the coding region, but there were three bases mutated in non-coding region behind stop codon. Probably, in the course of growth metabolism of Alexandium minutum, MAP executed different physiological functions and controlled the production of the toxin. In the meantime, map gene induced encoding products of the related poisonous gene, and enabled it has active expressions to starting toxin synthesis.

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Periodical:

Advanced Materials Research (Volumes 779-780)

Pages:

235-238

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.779-780.235

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Online since:

September 2013

Authors:

Li Wang, Jia Yuan Li, Qi Xu, Qing Ping Zhong, Zhen Lin Liao

Keywords:

Alexandrium minutum, Genome Library, Map, PSP

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References

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