Determination of Imidacloprid, Carbendazim and Thiabendazole Residues in Vegetables and Fruits by HPLC

Abstract:

The aim of this work was to establish a method that the residues of imidacloprid,carben-dazim and thiabendazole in fruits and vegetables were determined by HPLC.Acetonitrile was edded in the sample for extraction solvent, and using sodium chloride for absorbing water of the sample,then purified by NH₂ solid phase extraction cartridge, the sample was detected by HPLC.Under the selected conditions,the detection limits of imidacloprid, carbendazim, thiabendazole were 0.002 mg/kg,0.003 mg/kg and 0.003 mg/kg respectively.Tests for recovery were made by addition of three pesticidess standards at three different concentration levels (0.1, 0.5 and 1.0 mg/kg) to the blank sample.The mean recovery rates of imidacloprid, carbendazimand thiabendazole were87.0%~99.2%,82.0%~96.0% and 80.0%~95.0% respectively , and the relative standard deviations were 0.52%~6.80%.The sensitivity, accuracy and precision of this method were able to meet the requirements for pesticide residue analysis.

• Periodical: Advanced Materials Research (Volumes 781-784)
• Pages: 1392-1396
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.781-784.1392
• Online since: September 2013
• Authors: Ling Lin, Zheng Peng, Chun Liang Yang*, Ming Yue Wang, Yu Bing Zha, Li Li Liu, Shao Dong Zeng
• Keywords: Carbendazim, Fruits, High Performance Liquid Chromatography (HPLC), Imidacloprid, Residue, Thiabendazole, Vegetables

References

[1] Ying Chen, P.H. Cong J.Y. Nie, Zing Li, G.F. Xu, Determination of residues of carbendazim and thiabendazole pesticides in fruits by disper sive solid- phase extr action ( disper sive SPE) and HPLC ，J. Journal of Fruit Science, 25(2008)769-773.

[2] Q.X. Rao， M.Q. Qu, X.Y. Zhao, Z.T. Xing, Determination of carbendazim, thiabendazole and benomyl residues in vegetables and fruits by HPLC,J. Acta Agriculture Shanghai, 25(2009) 85-88.

[3] Cao Ren, Jing Nie, Na Sha, H.F. Lian, D.S. Wen, The optimization of the determination method of 7 pesticide residues in vegetable and fruit by using waters HPLC, J. Inner Mongolia agricultural science and technology, 2 (2012) 52-53.

[4] C.L. Liu, F.M. Liu, Li Li, R.H. Lan, S.R. Jiang, The determination of carbendazim and imidacloprid residues in asparagus by HPLC,J. Chinese Journal of Pesticide Science, 6 (2004), 93-96.

[5] D.F. Wang, L.H. Chen, Jun. You, Z.H. Huang, J.C. Zheng, R.L. Wang, Simultaneous determination of thiabendazole and carbendazim residues in fruits and vegetables by solid phase extraction and HPLC,J. Agrochemicals, 47(2008) 443-447.

[6] Attilio V, Giovanni V, Swizly A, Determination of carbendazim, thiabendazole and thiophanateme-thyl in banana(Musa acuminata) samples imported to Italy, J. Food Chemistry, 87 (2004) 383-386.

DOI: 10.1016/j.foodchem.2003.12.009

[7] Fernandez M, Rodriguez R, Pico Y, Liquid chromatographic-mass spectrometric determination of postharvest fungicides in citrus fruits, J. Chromatogr A, 912(2001)301-310.

DOI: 10.1016/s0021-9673(01)00576-3

[8] Y.J. Wu, Wei Zhu, Y.Y. Cheng, Determination of Carbendazim Residue in Dendrobium Candidum Wall. ex Lindl., Panax Quinquefolium and Their Preparations by HPLC-MS,J. Chinese Journal of Analytical Chemistry, 34(2006)235-238.

[9] Grujic S, Radisic M, Vasiljevic T, et a1. Determination of carbendazim residues in fruit juices by liquid chromatography-tandem mass spectrometry ,J. Food Addit Contaim, 22 (2005)1132-1137.

DOI: 10.1080/02652030500352863

[10] Z.Q. Kong, F.S. Dong, X.G. Liu, Jun Xu, Yong Gong, W.L. Shan,Y. Q Z, Determination of 4 Pestic- ides Residues in Citrus and Essential Oil by UPLC-MS,J. Chinese Journal of Analytical Chemistry, 40 (20 12)474-477.

DOI: 10.3724/sp.j.1096.2012.10700

[11] L.W. Yang, Determination of carbendazim residues by UV spectrophotometry method,J. Anhui chemical industry, 32(2006)70-71.