Investigation of the Interference of Carbon Nanomaterials with SYBR Green I-Based Real-Time PCR

Abstract:

Some carbon nanomaterials have been proved to be able to improve the PCR amplification efficiency. If used in quantitative real-time PCR (qPCR), these nanomaterials must be tested whether fluorescence processing is interfered after they are added in the PCR system. In this study, 76 different carbon nanomaterials were tested in SYBR Green I-based qPCR, and the results demonstrated that about half carbon nanomaterials tested in this study could alter the PCR amplification profile probably due to the fluorescence quenching. Surprisingly, lower concentrations of nanomaterials led to more slight interference with the melting temperature.

• Periodical: Advanced Materials Research (Volumes 785-786)
• Pages: 550-555
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.785-786.550
• Online since: September 2013
• Authors: Fu Ming Sang, Yuan Sun, Zhong Xu, Yu Shi Wang, Zhi Zhou Zhang*
• Keywords: Carbon Nanomaterials, Fluorescence Quenching, Melting Temperature, Real-Time PCR

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