Construction of Nano Coenzyme and Enzyme Multilayer Film on Gold Electrode by Self-Assembly Method

Kun Yun He; Xing Sheng Wang; Hai Tao Zheng; Shin Ichiro Suye

doi:10.4028/www.scientific.net/AMR.788.69

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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 788Construction of Nano Coenzyme and Enzyme...

Construction of Nano Coenzyme and Enzyme Multilayer Film on Gold Electrode by Self-Assembly Method

Abstract:

A ferrocene labeled high molecular weight coenzyme derivative (PEI-Fc-NAD) was prepared by attaching both ferrocene (Fc) and coenzyme (nicotinamide adenine dinucleotide, NAD⁺) to the backbone of poly (ethylenimine) (PEI). A NAD-dependent glucose-6-phosphate dehydrogenase (G6PDH) was used with PEI-Fc-NAD to fabricate a multilayer film on gold electrode by layer-by-layer self-assembly method. The G6PDH/PEI-Fc-NAD multilayer film was characterized by atom force microscope and electrochemical experiment, which proved that a nanostructure thin film was immobilized by the LBL technique. The modified electrode showed the current response to glucose-6-phosphate in the absence of free coenzyme, and kept about 85% response after storage in buffer for 2 month.

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Periodical:

Advanced Materials Research (Volume 788)

Pages:

69-72

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.788.69

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Online since:

September 2013

Authors:

Kun Yun He, Xing Sheng Wang, Hai Tao Zheng, Shin Ichiro Suye

Keywords:

Biosensor, Coenzyme, Dehydrogenase, Nanostructure

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