Effect of Silver Ions on the Structural and Antibacterial Properties of Bone Replacement Material

Abstract:

Antibacterial materials based on calcium phosphates have wide range of biomedical applications in the prevention of microbial infections. The synthesis of inorganic mineral component of bone i.e. hydroxyapatite was done with the addition of silver (Ag) (5-15 wt %) as antibacterial agent. The wet precipitation synthesis was carried out using diammonium hydrogen phosphate and calcium nitrate as P and Ca precursors. The presence and effect of silver addition on the structure was studied using Fourier Transform-Infrared (FTIR) spectroscopy and Energy Dispersive X-ray (EDX) techniques. The antibacterial properties of all samples were evaluated using Disc Diffusion Technique (DDT) against S. aureus, B. subtilis, P. aeruginosa and E. coli. Antibacterial activities of samples were found to vary depending on the bacterial species and Ag loading percentage. The antibacterial assay suggested that the addition of Ag ions within hydroxyapatite can be effectively provided the required level of antibacterial activity against bacteria.

• Periodical: Advanced Materials Research (Volume 795)
• Pages: 692-696
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.795.692
• Online since: September 2013
• Authors: Nida Iqbal, Mohammed Rafiq Abdul Kadir, Nasrul Humaimi Bin Mahmood, Micheal Moses, Mashitah Binti Mad Salim, Nik Ahmad Nazim Nik Malek
• Keywords: Antibacterial Activity, Hydroxyapatite (HA), Silver, Wet Precipitation

References

[1] A. Simchi, E. Tamjid, F. Pishbin and A.R. Boccaccini: Nanomed. Nanotech. Biol. Med Vol. 7 (2011), pp.22-39.

[2] V.D Sergey: Biomaterials Vol. 31 (2010), p.1465.

[3] E. Landi, F. Valentini and A. Tampieri: Acta. Biomater Vol. 4 (2008), P.1620.

[4] N. Iqbal, R. Nazir, A. Asif, A.A Chaudhry, M. Akram, G.Y Fan, A. Akram, R. Amin, S.H. Park and R. Hussain: Curr. Appl. Phys. Vol. 12 (2012), p.755.

DOI: 10.1016/j.cap.2011.11.003

[5] P.V. Budanov, O.R. Baev and Z.M Musaev: Int. J. Gynecol. Obstet Vol. 70 (2000), p.66.

[6] J. Mahan, D. Seligson, S. Henry, P. Hynes and J. Dobbins: Orthopedics Vol. 14 (1991), p.305.

[7] V. Krasimir, C. Jessica and J.G. Hans: expert. Rev. Med. Devices 6 (2009), pp.553-567.

[8] K. Yoshida, M. Tanagawa and M. Atsuta: J. Biomed. Mater. Res., 47 (1999), p.516–522

[9] V.K. Sharma, R.A. Yngard and Y. Lin: Adv. Colloid Interface Vol. 145 (2009), pp.83-96.

[10] N. Jones, B. Ray, K.T. Ranjit and A.C. Manna: FEMS Microbiol. Lett. Vol. 279, ( 2008), p.1–76.

[11] Y. Liu, D. Hou and G. Wang: Mater. Chem. Phys Vol. 86 (2004), p.69.

[12] J. Klinkaewnarong , E. Swatsitang and S. Maensiri: J. Nanosci. Nanotechnol. Vol. 11 (2011), p.8831.

[13] S.K. Padmanabhan, B. Avinash, C.C. Min, V.J. Lee, T.N. Kim and S-J. Cho: Particuology Vol. 7(2009), p.466.