Study on Silk Fibroin D-Mannose Blend Films

Abstract:

The preparation and properties of different proportion of D-mannose-fibroin membrane were studied in this paper. The change of crystal structure of silk fibroin (SF) in the process of becoming insoluble by adding D-mannose was studied. The blend silk fibroin films were casted by silk fibroin solutions mixing with D-mannose at a series of ratios at room temperature. The solubility, structure, and mechanical properties of the blend films were measured. The results show that with the content of D-mannose increasing, the solubility decreases. The blend films became insoluble when the mass ratios of D-mannose /SF are more than 1/10. The crystalline structure of SF is mostly changed into Silk I and there was almost no Silk II structure. Mechanical properties indicated that D-mannose could significantly improve the flexibility of silk films. With the good transmittance, D-mannose/SF blend membrane can be used in corneal tissue engineering.

• Periodical: Advanced Materials Research (Volume 796)
• Pages: 112-116
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.796.112
• Online since: September 2013
• Authors: Jing Hou, Qiao Fen Qian, Shan Shan Zhang, Yi Yun Shen, Shen Zhou Lu
• Keywords: Blend Film, Corneal, D-Mannose, Silk Fibroin, Transparent

References

[1] C.J. Doillon, M.A. Watsky, M. Hakim, et al, A collagen-based scaffold for a tissue engineered human cornea: physical and physiological properties, Int J Artif Organs. 26(2003) 764–773.

DOI: 10.1177/039139880302600810

[2] JS Choi, JK Williams, M Greven, et al, Bioengineering endothelialized neo-corneas using donor-derived corneal endothelial cells and decellularized corneal stroma, Biomaterials. 31(26) (2010) 6738–6745.

DOI: 10.1016/j.biomaterials.2010.05.020

[3] A.E. Grau, J.A. Durán, Treatment of a Large Corneal Perforation With a Multilayer of Amniotic Membrane and TachoSil, Cornea. 31(1) (2012) 98-100.

DOI: 10.1097/ico.0b013e31821f28a2

[4] Y. Hashimoto, S. Funamoto, S. Sasaki, T. Honda, S. Hattori, K. Nam, Preparation and characterization of decellularized cornea using high-hydrostatic pressurization for corneal tissue engineering, Biomaterials. 31(2010) 3941-3948.

DOI: 10.1016/j.biomaterials.2010.01.122

[5] P. Fagerholm, N.S. Lagali, K. Merrett, et al, A biosynthetic alternative to human donor tissue for inducing corneal regeneration: 24-month follow-up of a phase 1 clinical study, Transl Med. 2(46) (2010) 46-61.

DOI: 10.1126/scitranslmed.3001022

[6] L.J. Bray, K.A. George, S.L. Ainscough, D.W. Hutmacher, T.V. Chirila, D.G. Harkin, Silk Film Culture System for in vitro Analysis and Biomaterial Design, Biomaterials. 32(22) (2011) 5086-5091.

DOI: 10.1016/j.biomaterials.2011.03.068

[7] B.D. Lawrence, J.K. Marchant, M.A. Pindrus, F.G. Omenetto, D.L. Kaplan, Silk film biomaterials for cornea tissue engineering, Biomaterials. 30 (2) (2009) 1299–1308.

DOI: 10.1016/j.biomaterials.2008.11.018

[8] P.W. Madden, J.N.X. Lai, K.A. George, T. Giovenco, D.G. Harkin, T.V. Chirila, Human corneal endothelial cell growth on a silk fibroin membrane, Biomaterials. 32(17) (2011) 4076-4084.

DOI: 10.1016/j.biomaterials.2010.12.034

[9] M.Z. Li, S.Z. Lu, Z.Y. Wu, Study on Porous Silk Fibroin Materials: 1. Fine Structure of Freeze-dried Silk Fibroin, Journal of Polymer Science. 79(2001) 2185-2191.

DOI: 10.1002/1097-4628(20010321)79:12<2185::aid-app1026>3.0.co;2-3

[10] W. Zhou, X. Chen, Z.Z. Shao, Conformation Studies of Silk Proteins with Infrared and Raman Spectroscopy, Progress in Chemistry. 18 (11) (2006) 1514-1522.

[11] Y. Zeng, J. Yang, K. Huang, et al, A comparison of biomechanical properties between human andporcine cornea, Journal of Biomechanics. 34(2001) 357-365.

[12] T. J. T. P. Van Den Berg, K. E. W. P. Tan, Light Transmittance of the Human Cornea from 320 to 700 nm for Different Ages, Vision Research. 34(11) (1994) 1453-1456.

DOI: 10.1016/0042-6989(94)90146-5