Enzyme-Catalyzed Synthesis of Microalgae-Derived Biodiesel Using a Chemically Modified Immobilized Lipase

Abstract:

Optimized synthesis conditions for biodiesel from microalgae oil and methanol using a modified immobilized lipase from Candida sp. as catalyst were investigated. The methanol tolerance of the immobilized lipase modified with succinic anhydride was determined. The results showed that the modified enzyme exhibited higher stability in methanol as compared with the unmodified enzyme. Then, the modified immobilized enzyme was applied to synthesize biodiesel using methanol and microalgae oil from Chlorella sp. Cells as substrates and the synthesis conditions were optimized. Tert-butanol was the most suitable organic solvent for biodiesel synthesis. The molar ratio of the reactants was found to be an important parameter affecting the yield of biodiesel. When the molar ratio of the methanol to Chlorella oil is 5:1, the yield of biodiesel catalyzed by modified lipase reaches the maximum of 92.5%. While the native immobilized Candida sp. lipase is used as catalyst, the yield of biodiesel get the maximum of 82.4% at the methanol / Chlorella oil molar ratio of 4. The reuse stabilities of the modified enzyme and unmodified enzyme were also studied in the present work. These experimental results proved that the immobilized lipase modified with succinic anhydride was suitable for the synthesis of microalgae-derived biodiesel.

• Periodical: Advanced Materials Research (Volumes 805-806)
• Pages: 176-181
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.805-806.176
• Online since: September 2013
• Authors: Chun Hua Yin, Na Miao, Shou Li Yuan, Xue Dong, Hai Yan
• Keywords: Bio-Diesel, Lipase, Microalgae, Modification

References

[1] J. Kansedo, K.T. Lee, S. Bhatia, Biodiesel production from palm oil via heterogeneous transesterification, Biomass Bioenerg. 33 (2009) 271–276.

DOI: 10.1016/j.biombioe.2008.05.011

[2] D. Rattanaphra, P. Srinophakun, Biodiesel production from crude sunflower oil and crude jatropha oil using immobilized lipase, J. Chem. Eng. Jpn. 43 (2010) 104–108.

DOI: 10.1252/jcej.09we010

[3] M. Canakci, The potential of restaurant waste lipids as biodiesel feedstocks, Bioresour. Technol. 98 (2007) 183–190.

DOI: 10.1016/j.biortech.2005.11.022

[4] M. Canakci, A.N. Azsezen, E. Arcaklioglu, A. Erdil, Prediction of performance and exhaust emissions of a diesel engine fueled with biodiesel produced from waste frying palm oil, Expert. Syst. Appl. 36 (2009) 9268–80.

DOI: 10.1016/j.eswa.2008.12.005

[5] Y. Chisti, Biodiesel from microalgae, Biotechnol. Adv. 25 (2007) 294–306.

[6] T.M. Mata, A.A. Martins, N.S. Caetano, Microalgae for biodiesel production and other applications: A review, Renew. Sust. Energ. Rev. 14 (2010) 217–232.

[7] A.L. Ahmad, N.H. Mat Yasin, C.J.C. Derek, J.K. Lim, Microalgae as a sustainable energy source for biodiesel production: A review, Renew. Sust. Energ. Rev. 15 (2011) 584–593.

DOI: 10.1016/j.rser.2010.09.018

[8] Y. Shimada, Y. Watanabe, T. Samukawa, A. Sugihara, H. Noda, H. Fukuda, Y. Tominaga, Conversion of vegetable oil to biodiesel using immobilized Candida antarctica lipase, J. Am. Oil Chem. Soc. 76 (1999) 789–793.

DOI: 10.1007/s11746-999-0067-6

[9] J. Lu, L. Deng, R. Zhao, R. Zhang, F. Wang, T. Tan, Pretreatment of immobilized Candida sp. 99-125 lipase to improve its methanol tolerance for biodiesel production, J. Mol. Catal. B-Enzym. 62 (2010) 15–18.

DOI: 10.1016/j.molcatb.2009.08.002

[10] H. Yan, J. Zhou, H. He, and Y. Wei, J Sun, isolation and heterotrophic culture of chlorella sp., J. Univ. Sci. Technol. Beijing (in Chinese), 27 (2005)408-412.

[11] H. Yan, H. He, C. Yin, X. Jia, P. Xu, X. Liu, J. Hu, Z. Jiang, and X. Zhang, Alkali-catalyzed transesterification of lipid from Chlorella sp. USTB-01 to biodiesel, J. Biobased Mater. Bio. 5 (2011) 1–6.

DOI: 10.1166/jbmb.2011.1151

[12] C. Yin, C. Zhang, M. Gao, Enzyme-catalyzed synthesis of vitamin E succinate using a chemically modified Novozym-435, Chinese J. Chem. Eng. 19 (2011) 135-139.

DOI: 10.1016/s1004-9541(09)60189-0

[13] M. Gargouri, M.D. Legoy, Bienzymatic reaction for hydroperoxide production in a multiphasic system, Enzyme Microb. Tech. 21 (1997) 79-84.

DOI: 10.1016/s0141-0229(96)00229-3

[14] A. Szabo, M. Kotorman, I. Laczko, L.M. Simon, Improved stability and catalytic activity of chemically modified papain in aqueous organic solvents, Process Biochem. 44 (2009) 199-204.

DOI: 10.1016/j.procbio.2008.10.008

[15] C. Lanne, S. Boeren, K. Vos, C. Veeger, Rules for optimization of biocatalysis in organic solvents, Biotechnol. Bioeng. 30 (1987) 81-87.

DOI: 10.1002/bit.260300112

[16] L.S. Gorman, J.S. Dordick, Organic solvents strip water of enzymes, Biotechnol. Bioeng. 38 (1992) 75-81.

DOI: 10.1002/bit.260390405