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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 805-806Characterization and Determination of...

Characterization and Determination of Acetyl-Coenzyme a Carboxylase (ACCase) from Eukaryotic Alga Chlorella Sp. USTB-01

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Abstract:

In the present study, the Acetyl-coenzyme A carboxylase (ACCase) of Chlorella sp. USTB-01 purified and characterized. Molecular weight of ACCase was calculated to be about 456 kD and was comprised of two identical subunits of 229 kD. The enzymatic characteristics isoelectric point was 6.7. Moreover, a rapid and sensitive assay of High performance liquid chromatography (HPLC) was established to measure overall activity of ACCase in low protein content condition by detect the ACCase activity by measuring ADP production and Acetyl-coenzyme A (Acetyl-CoA).

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Periodical:

Advanced Materials Research (Volumes 805-806)

Pages:

255-259

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.805-806.255

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Online since:

September 2013

Authors:

Hai Ou Wang, Yang Jing, Zhe Tang

Keywords:

Acetyl-Coenzyme a Carboxylase, Characterization, Determination, Electrophoresis, High Performance Liquid Chromatography (HPLC)

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© 2013 Trans Tech Publications Ltd. All Rights Reserved

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