Low Molecular Weight Branched Polyethylenimine-Graft-Lentinan as a Novel Synthetic Gene Carrier

Abstract:

LMW Branched Polyethylenimine-graft-lentinan was synthesized by performing crosslinker CDI in the presence of water-soluble Polysaccharide (Mw 70,000) and polyethylenimine (PEI, Mw 600). The chemistry of the PEI-g-lentinan obtained were characterized. The results indicated that the amines of lentinan were grafted with PEI. Gel electrophoresis showed that DNA migration was retarded completely at a N/P ratio of 10/1, indicating good DNA condensation capability of PEI-g-lentinan. The cytotoxicity of PEI-g-lentinan was evaluated, and the results reflected that PEI-g-lentinan had a lower cytotoxicity than PEI (25 K). Gene transfection efficiency of PEI-g-chitosan in cos-7 cells was determined. Remarkably,PEI-g-lentinan showed a higher transfection efficiency than that of PEI (25 K) in vitro.

• Periodical: Advanced Materials Research (Volume 815)
• Pages: 293-298
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.815.293
• Online since: October 2013
• Authors: Li Jing Min, Jing Fen Li
• Keywords: Lentinan, Polyethylenimine, Synthetic Gene Carrier

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