Combined Effects of Bone Marrow-Derived Mesenchymal Stem Cells and PLGA-PEG-PLGA Scaffolds on Repair of Articular Cartilage Defect

Ching Wen Hsu; Ping Liu; Song Song Zhu; Feng Deng; Bi Zhang

doi:10.4028/www.scientific.net/AMR.815.345

Paper Titles

Fabrication of Calcium Sulfate/Tri-Calcium Phosphate Composite Bone Fillers through Molding Method
p.325

Corn-Rice Powder Extrusion Key Parameters in Twin-Screw Extrusion Process
p.329

Study on the Antibacterial Performance of the Antibacterial Shutter Blades
p.333

Research on Corn Stover Fermentation by Multi-Bacterium and Improvement of Fermentation Devices
p.339

Combined Effects of Bone Marrow-Derived Mesenchymal Stem Cells and PLGA-PEG-PLGA Scaffolds on Repair of Articular Cartilage Defect
p.345

Inhibition of Recombinant Chicken Cystatin on Expression of Aflatoxin Biosynthesis Genes on Peanut
p.350

Preliminary Researches on Possibility of Several Kinds of Plants Including Dracaena Angustifolia Used to Relieve the Damages Caused by Organic Phosphorous Compounds
p.358

Preliminary Researches on Conditions to Extract Chlorophyll from Leaves of Wedelia Trilobata (L.) Hitchc
p.362

Preparation and Characterization of Hexadecane Microcapsule Phase Change Materials by In Situ Polymerization
p.367

HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 815Combined Effects of Bone Marrow-Derived...

Combined Effects of Bone Marrow-Derived Mesenchymal Stem Cells and PLGA-PEG-PLGA Scaffolds on Repair of Articular Cartilage Defect

Abstract:

Here we reported a combined technique for articular cartilage repair, consisting of bone arrow mesenchymal stem cells (BMMSCs) and poly (dl-lactide-co-glycolide-b-ethylene glycol-b-dl-lactide-co-glycolide) (PLGA-PEG-PLGA) triblock copolymers carried with tissue growth factor (TGF-belat1). In the present study, BMMSCs seeded on PLGA-PEG-PLGA with were incubated in vitro, carried or not TGF-belta1, Then the effects of the composite on repair of cartilage defect were evaluated in rabbit knee joints in vivo. Full-thickness cartilage defects (diameter: 5 mm; depth: 3 mm) in the patellar groove were either left empty (n=18), implanted with BMMSCs/PLGA (n=18), TGF-belta1 modified BMMSCs/PLGA-PEG-PLGA. The defect area was examined grossly, histologically at 6, 24 weeks postoperatively. After implantation, the BMMSCs /PLGA-PEG-PLGA with TGF-belta1 group showed successful hyaline-like cartilage regeneration similar to normal cartilage, which was superior to the other groups using gross examination, qualitative and quantitative histology. These findings suggested that a combination of BMMSCs/PLGA-PEG-PLGA carried with tissue growth factor (TGF-belat1) may be an alternative treatment for large osteochondral defects in high loading sites.