Fabrication of Thermoresponsive Glycopolymers Fibers for Lectin Recognition

Abstract:

A thermoresponsive glycopolymer poly-(N-isopropylacrylamide-co-6-O-vinylsebacoyl D-glucose) (poly-NIPAM-co-VSEG; PNE) has been prepared by free radical polymerization process, and subsequently processed into nanofibers with poly-L-lactide-co-ε-caprolactone (PLCL) using electrospinning. The thermoresponsive glycopolymer materials have been characterized by Fourier transform infrared (FTIR) spectroscopy, ¹H NMR, and scanning electron microscopy (SEM). And the lower critical solution temperature (LCST) of these thermoresponsive materials was also measured. The nanofibers containing PNE can greatly selective recognize with lectin Concanavalin A (Con A), and the adsorbed Con A can be easily eluted by 1 M glucose solution. These results indicate that PNE can separate and purify proteins, and also the temperature sensitive characteristic has the potential to achieve the intelligent control.

• Periodical: Advanced Materials Research (Volumes 821-822)
• Pages: 981-985
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.821-822.981
• Online since: September 2013
• Authors: Lei Wang, Jing Quan, Li Min Zhu
• Keywords: Copolymerization, Glycopolymer, Lectin Recognition, Thermosensitive

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