A Rapid Quantitative Analysis of Native Antioxidants in Natural Rubber (Hevea brasiliensis) During Maturation

Abstract:

Natural rubber (NR) obtained from H. brasiliensis is known to be susceptible to oxidative degradation according to the amount of double bonds in the structure of the polymer, i.e. poly (cis-1,4-isoprene) [1]. However NR has been reported to contain native antioxidants such as phytosterols, phospholipids and tocotrienols [2]. Among those, γ-tocotrienol, present in hevea latex was reported to exhibit “in-vitro” antioxidant activity [3]. However the direct involvement of γ-tocotrienol or other antioxidants naturally present in NR in the protection of NR against oxidation is difficult to assess as the works were carried out with different of rubber types and in different conditions [4,5]. In the framework of a work on the dynamics of γ-tocotrienols in NR samples obtained from coagula maturated during several durations (0 to 15 days) in parallel with measurement of standard properties such as P₀ and PRI, methodological development of the analysis of tocotrienols and derivatives is presented in this study.HPLC-MS has been shown to be an accurate technique for lipid analysis in NR [6]. However, this is a time-consuming technique especially with large number of samples due to a necessary step of saponification. Moreover, the structures of γ-tocotrienol and its dimers were found to be affected by the strong alkali condition of saponification (data not shown). Therefore a rapid quantitative method for γ-tocotrienol from NR using high performance thin layer chromatography (HP-TLC) has been developed. Lipid extracts from NR samples could be simply analyzed by HP-TLC without any derivatization and the detectable quantity could be in nanogram range. Statistical analysis of the data showed that the method is precise, accurate, reproducible and sensitive. Thus the proposed HP-TLC method can be successfully used for the quantification of γ-tocotrienol from NR samples. This technique will be useful to conduct further experiments on antioxidant activity of NR lipids and to relate the results with physical properties of NR, especially those linked to resistance to oxidation.

• Periodical: Advanced Materials Research (Volume 844)
• Pages: 410-414
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.844.410
• Online since: November 2013
• Authors: Natedao Musigamart, Siriluck Liengprayoon, Sriroth Klanarong, Eric Dubreucq, Jerome Lecomte, Laurent Vaysse
• Keywords: Antioxidant, Hevea brasiliensis, High Performance Thin Layer Chromatography, HPTLC, Natural Rubber (NR), γ-Tocotrienol

References

[1] L.H. Gan, K.K. Chee, Kinetic study of oxidative scission in natural rubber, Rubb. Chem. Technol. 65 (1991) 1-4.

[2] H. Hasma, A.B. Othman, Role of some non-rubber constituents on thermal oxidative ageing of natural rubber, J. Nat. Rubb. Res. 5 (1990) 1-8.

[3] A. KamaI-Eldin, L.A. Appelqvist, The chemistry and antioxidant properties of tocopherols and tocotrienols, Lipids 31 (1996) 671-701.

DOI: 10.1007/bf02522884

[4] M. Nadarajah, A. S. L. Tirimanne, A. Coomarasamy, S. K. Asinathan, Some naturally occurring antioxidants in Hevea brasiliensis latex, Q. Jl. Rubb. Ret. Inil. Ceylon 48 (1971) 202-211.

[5] M. Boucher, G. Carlier, Effect of non-rubber ingredients on ageing, Rev. gen. Caoutch. 41 (1964) 1297-1308.

[6] S. Liengprayoon, J. Chaiyut, K. Sriroth, F. Bonfils, J. Sainte Beuve, E. Dubreucq, L. Vaysse, Lipid compositions of latex and sheet rubber from H. brasiliensis depend on clonal origin,: European Journal of Lipid Science and Technology (2013).

DOI: 10.1002/ejlt.201300023

[7] H. C. Andola, V. K. Purohit, High Performance Thin Layer Chromatography (HPTLC): A Modern Analytical tool for Biological Analysis, Nature and Science 8 (2010) 58-61.

[8] P. Rashmini, P. Mrunali, D. Nitin, D. Nidhi, P. Bharat, HPTLC Method Development and Validation: Strategy to Minimize Methodological Failures, JFDA. 20 (2012) 794-804.

[9] S. Liengprayoon, F. Bonfils, J. Sainte Beuve, K. Sriroth, E. Dubreucq, L. Vaysse, Development of a new procedure for lipid extraction from Hevea brasiliensis natural rubber, Eur. J. Lipid Sci. Technol. 6 (2008) 563-569.

DOI: 10.1002/ejlt.200700287

[10] B. Fuchs, R. Süß, K. Teuber, M. Eibisch, J. Schiller, Lipid analysis by thin-layer chromatography; A review of the current state, J. Chromatogr. A. 1218 (2011) 2754-2774.

DOI: 10.1016/j.chroma.2010.11.066

[11] J. Intapun, Study of the effect of biological maturation of coagula of Hevea brasiliensis latex on dry rubber properties, Thesis (2009).

[12] C. K. Chow, H. H. Draper, Isolation of γ-tocotrienol dimers from Hevea latex, BIOCHEMISTRY. 9 (1970) 445-450.

DOI: 10.1021/bi00804a036