Bone Marrow Cell Response on Carbonate Apatite/PCL-Coated α-Tricalcium Phosphate Foam

Abstract:

The aim of this research work was to investigate in vitro effect of the carbonate apatite/poly (ε-caprolactone) (CO₃Ap/PCL) on α-tricalcium phosphate (α-TCP) foam was produced by sintering CaCO₃ and CaHPO₄2H₂O at 1500°C for 5 h. It was then coated with carbonate apatite (CO₃Ap)/Poly-ε-caprolactone (PCL) (wt/wt=1/3) to improve both mechanical and biological properties. The initial cell attachment and proliferation of the bone marrow cells were carried out on the α-TCP and CO₃Ap/PCL-coated α-TCP foams. The cell proliferation was calculated by AlamarBlue assay. The cells were able to migrate and proliferate well on both α-TCP and CO₃Ap/PCL-coated α-TCP foams indicating an excellent biocompatibility. The incorporation of CO₃Ap on the coating layer improved cellular attachment and accelerated proliferation. Thus, CO₃Ap/PCL-coated α-TCP foam might be a promising candidate as implant material.

• Periodical: Advanced Materials Research (Volume 858)
• Pages: 7-12
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.858.7
• Online since: November 2013
• Authors: Thi Bang Le, Xing Ling Shi, Ishikawa Kunio, Radzali Othman
• Keywords: Biological Response, Bone Marrow Cell, Carbonate Apatite, Tricalcium Phosphate

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