Determination of Content of the Four Alkaloids and the Total Alkaloid in Co-Dergocrine Mesyiate Tablets by HPLC

Hong Kun Yue; Ming Chang; Si Jie Liu; Ran Zhou

doi:10.4028/www.scientific.net/AMR.884-885.419

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 884-885Determination of Content of the Four Alkaloids and...

Determination of Content of the Four Alkaloids and the Total Alkaloid in Co-Dergocrine Mesyiate Tablets by HPLC

Abstract:

Objective: To determine the four kinds of alkaloid and the total alkaloid in Co-dergocrine Mesyiate tablets by HPLC. Methods: HPLC was used. C18column (250mm×4.6mm5μm) was selected, the mobile phase was acetonitrile-H₂O-triethylamine (50:150:5).Detection was performed by UV with wavelength of 280nm.Results:A linearity of alkaloid I was obtained from 240.336 to 961.344μg/mL with good correlation (r=0.9997). The average recovery of alkaloid Iwas 101.16%, and RSD was0.92%. A linearity of alkaloidII(α isomer) was obtained from 240.336 to 961.344μg/mL with good correlation (r=0.9998). The average recovery of α-alkaloidII was 99.31%, and RSD was1.14%. A linearity of alkaloidII(β isomer) was obtained from 240.336 to 961.344μg/mL with good correlation (r=0.9999). The average recovery of β-alkaloidII was 97.31%, and RSD was1.96%. A linearity of alkaloid IIIwas obtained from 240.336 to 961.344μg/mL with good correlation (r=0.9998).The average recovery of alkaloidIII was 102.02%, and RSD was 1.38%.Conclusion:The HPLC is sensitive and accurate in determining the four kinds of alkaloid in Co-dergocrine Mesyiate tablets. It can be applied efficiently.