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Microbial Production of 1,3-Propanediol by a Newly Isolated Citrobacter freundii Strain CF-5

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Abstract:

Microorganisms tolerant to 1, 3-propanediol (1, 3-PDO) were screened out from various environmental samples, and isolate CF-5 was found to produce 2.8 g/L 1, 3-PDO with glycerol as carbon source. Based on 16S rRNA gene sequence analysis, CF-5 was identified as a Citrobacter freundii strain. The fermentation conditions of strain CF-5 were analyzed. Higher biomass production was obtained in the medium supplemented with limited amount of glucose without repression of conversion efficiency of glycerol to 1, 3-PDO. Under the combined aerobic and oxygen limited incubation conditions, higher yield of 1, 3-PDO was reached. The fed-batch fermentation was performed in a 5 L bioreactor, 1, 3-PDO was found mainly produced in exponential growth phase cells of C. freundii CF-5 with the highest productivity 0.63 g/L*h, and the overall yield of 1, 3-PDO fermentation was 11.8 g/L within 36 h fermentation.

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Periodical:

Advanced Materials Research (Volumes 884-885)

Pages:

459-464

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.884-885.459

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Online since:

January 2014

Authors:

Yue Yu, Li Tao Shi, Wen Yi Guo, Hong Jiang Yang

Keywords:

3-Propanediol, Citrobacter Freundii, Fed-Batch Fermentation

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© 2014 Trans Tech Publications Ltd. All Rights Reserved

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