Low Temperature and Copper Induce Viable but Nonculturable State of Salmonella typhi in the Bottled Drinking Water

Abstract:

Salmonella in the normal state were injected into the sterile bottled drinking water with the concentration of 107CFU/ml finally. Then these samples were respectively treated by 4°C, -20°C, copper, the two latter samples were found that their numbers of bacteria colonies reduced to zero on the solid culture medium and the absorbance had no change in the liquid culture medium, they had no reproduction and can not be cultured, which had been thought that the cells were all dead. The ways of alteration in temperature, adding of tween20 and catalase may help awake up their culturability in the experiment. The samples had entered the so-called viable but nonculturable state (VBNC).

• Periodical: Advanced Materials Research (Volume 893)
• Pages: 492-495
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.893.492
• Online since: February 2014
• Authors: Lu Jiang*
• Keywords: Bottled Drinking Water, Induce, VBNC

References

[1] Jolivet-Gougeon, A., et al., Virulence of viable but nonculturable S. Typhimurium LT2 after peracetic acid treatment, International journal of food microbiology 112. 2 (2006) 147-152.

DOI: 10.1016/j.ijfoodmicro.2006.06.019

[2] Dwidjosiswojo, Zenyta, et al., Influence of copper ions on the viability and cytotoxicity of Pseudomonas aeruginosa under conditions relevant to drinking water environments, International journal of hygiene and environmental health 214. 6 (2011).

DOI: 10.1016/j.ijheh.2011.06.004

[3] Xu, Huai-Shu, et al., "Survival and viability of nonculturable Escherichia coli and Vibrio cholerae in the estuarine and marine environment, Microbial Ecology 8. 4 (1982) 313-323.

[4] Heim, Sabina, et al., The viable but nonculturable state and starvation are different stress responses of Enterococcus faecalis, as determined by proteome analysis, Journal of bacteriology 184. 23 (2002) 6739-6745.

DOI: 10.1128/jb.184.23.6739-6745.2002

[5] Guillou, Sandrine, et al., Survival of Campylobacter jejuni in mineral bottled water according to difference in mineral content: Application of the Weibull model, Water research 42. 8 (2008)2213- -2219.

DOI: 10.1016/j.watres.2007.11.035

[6] Buck, Alan, and James D. Oliver, Survival of spinach-associated Helicobacter pylori in the viable but nonculturable state, Food Control 21. 8 (2010) 1150-1154.

DOI: 10.1016/j.foodcont.2010.01.010

[7] Panutdaporn, N., et al., Resuscitation of the viable but non-culturable state of Salmonella enterica serovar Oranienburg by recombinant resuscitation-promoting factor derived from Salmonella Typhimurium strain LT2, International journal of food microbiology 106. 3 (2006).

DOI: 10.1016/j.ijfoodmicro.2005.06.022

[8] Oliver, James D., Recent findings on the viable but nonculturable state in pathogenic bacteria, FEMS microbiology reviews 34. 4 (2010) 415-425.

DOI: 10.1111/j.1574-6976.2009.00200.x