Study on the Process of the Reaction Catalyzed by Polyphenol Oxidase from Purple Sweet Potato

Abstract:

The polyphenol oxidase (PPO) was extracted from fresh purple sweet potato (PSP) by phosphate buffer solution, and spectrophotometry method was applied in the experiment. The process of the reaction catalyzed by PPO with different substrate concentrations and the relationship between enzyme concentrations and PPO activity were mainly studied here. The result showed that the effect of enzyme concentration on PPO activity was stronger than that of substrate concentration on PPO activity.

• Periodical: Advanced Materials Research (Volume 898)
• Pages: 153-156
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.898.153
• Online since: February 2014
• Authors: Lu Gao, Li Chun Zhao*, Ji Dong Duan, Yan Li Tao
• Keywords: Polyphenol Oxidase, Purple Sweet Potato, Substrate Concentration

References

[1] Mermelstein; Neil H. Fresh-cut produce quality & safety. Food Technology, 2012, 66 (7): 66-69.

[2] Chinwang U.; Siriphanich J. Enzymatic browning of fresh-cut galangal and its relationship to qxidative enzymes. J. of the Japanese Society for Horticultural Science, 2011, 1: 103-112.

DOI: 10.2503/jjshs1.80.103

[3] Du J. H. ；FU Y. C.; Wang N. Y. Effects of aqueous chlorine dioxide treatment on browning of fresh-cut lotus root. Food Sci. and Technol. 2009, 2: 654-659.

DOI: 10.1016/j.lwt.2008.08.007

[4] Chisari M.; Barbagallo R. N.; Spagna G. Characterization and role of polyphenol oxidase and peroxidase in browning of fresh-cut melon. J. Agric. Food Chem., 2008, 1: 132-138.

DOI: 10.1021/jf0721491

[5] Alfred M. M.; Eitan H. Polyphenol oxidase in plants. Phytochemistry, 1979, 18: 193-215.

[6] Finkle, B. J.; Nelson, R. F. Enzyme reactions with phenolic compounds: Effect of O-methyltransferase on a natural substrate of fruit polyphenol oxidase. Nature. 1963, 197, 902-903.

DOI: 10.1038/197902a0

[7] Peng Q.; Gao Y. X.; Yuan F. Research progress on purple sweet potato and its anthocyanins. Food Sci. 2010, 23: 401-405.

[8] Kume Akitomo. Food material derived from purple sweet potato and having blood circulation-improving effect. JP, 2001145471, (2001).

[9] Liu D.; Qian J. Y.; Wong L. Z. The efficiency of water soluable purple pigment of sweet potato as DPPH radical scavenger. Food Additive, 2003, 12: 50-53.

[10] Ozoglu H. Inhibition of enzymic browning in cloudy apple juice with selected antibrowning agents. Food Control, 2002, 23: 213.

DOI: 10.1016/s0956-7135(02)00011-7

[11] Simon, R. R.; Phillips, K. M. Vitamin D mushrooms: comparison of the composition of button mushrooms treated postharvest with UVB light or sunlight. J. Agric. Food Chem. 2011, 59, 8724-8732.

DOI: 10.1021/jf201255b

[12] Coetzer. Control of enzymatic browning in potato by sense and antisense RNA from tomato polyphenol oxidase. Agric. Food Chem., 2001, 49(2): 652.

DOI: 10.1021/jf001217f

[13] Duangmal K. A comparative study of PPO from taro and potato. Food Chem., 1999, 64: 351.

[14] Kacem B.; Cornel J. A. Nonenzymatic browning in aseptically package drinks: Effect of ascorbic acid, amino acid and oxygen. Food Sci. 1987, 52: 1668-1672.

DOI: 10.1111/j.1365-2621.1987.tb05902.x

[15] Franceco P. Inhibition of apple polyphenol oxidase by ascorbic acid, citric acid and sodium chloride. Food Proc and Pres. 1993, 17: 21-30.