Galactose Consuming Microbes for Ethanol Production from Seaweed

Abstract:

Gracilaria sp. is one of macroalgae that contain high amount of sugar especially galactose. This galactose can be fermented into bioethanol by galactose-consuming microbes similar to the widely known ethanol production by yeast S.cerevisae. Hence the main objective of this study is to isolate galactose consuming microbes which capable of fermenting galactose into bioethanol. For this purpose, microbes-containing sample from seaweed culture were grown on galactose agar and tested for their survival as well as ethanol production capability. Seven isolated microbes belong to fungi and bacteria species were tested for their capability in fermenting galactose to produce ethanol. The concentrations of ethanol that have been produced by isolated microbes were analyzed by dichromate method whereas the consumption of galactose was determined by Dinitrosalicyclic acid (DNS) method. It was found that ethanol production resulted from fermentation by S1, S2, S3 and S4 were 0.80% (w/v), 0.74% (w/v), 0.81% (w/v) and 0.85% (w/v) respectively. Identification of these strains, as well as optimization of their ethanol fermentation are undergoing in our laboratory.

• Periodical: Advanced Materials Research (Volume 925)
• Pages: 219-222
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.925.219
• Online since: April 2014
• Authors: Nur Zatul 'Iffah Zakaria, Dachyar Arbain*, Mohd. Noor Ahmad, Mohd Irfan Hatim Mohamed Dzahir
• Keywords: Bacteria, Bioethanol, Fungi, Seaweed

References

[1] U. Larsen, T. Johansen, and J. Schramm, Ethanol as a Fuel for Road Transportation, (2009).

[2] C. S. Goh and K. T. Lee, A visionary and conceptual macroalgae-based third-generation bioethanol (TGB) biorefinery in Sabah, Malaysia as an underlay for renewable and sustainable development, Renewable and Sustainable Energy Reviews, 14 (2010).

DOI: 10.1016/j.rser.2009.10.001

[3] X. Wang, X. Liu, and G. Wang, Two-stage hydrolysis of invasive algal feedstock for ethanol fermentation., Journal of integrative plant biology, 53 (2011) 246–52.

DOI: 10.1111/j.1744-7909.2010.01024.x

[4] J. Singh and S. Gu, Commercialization potential of microalgae for biofuels production, Renewable and Sustainable Energy Reviews, 14 (2010) 2596–2610.

DOI: 10.1016/j.rser.2010.06.014

[5] S. G. Wi, H. J. Kim, S. A. Mahadevan, D. -J. Yang, and H. -J. Bae, The potential value of the seaweed Ceylon moss (Gelidium amansii) as an alternative bioenergy resource., Bioresource technology, 100 (2009) 6658–6660.

DOI: 10.1016/j.biortech.2009.07.017

[6] H. Andriamanantoanina, G. Chambat, and M. Rinaudo, Fractionation of extracted Madagascan Gracilaria corticata polysaccharides: Structure and properties, Carbohydrate Polymers, 68 (2007) 77–88.

DOI: 10.1016/j.carbpol.2006.07.023

[7] M. H. Huesemann, L. Kuo, L. Urquhart, G. A. Gill, and G. Roesijadi, Acetone-butanol fermentation of marine macroalgae, Bioresource Technology, 108 (2012) 305–309.

DOI: 10.1016/j.biortech.2011.12.148

[8] S. S. Dhaliwal, H. S. Oberoi, S. K. Sandhu, D. Nanda, D. Kumar, and S. K. Uppal, Enhanced ethanol production from sugarcane juice by galactose adaptation of a newly isolated thermotolerant strain of Pichia kudriavzevii., Bioresource technology, 102 (2011).

DOI: 10.1016/j.biortech.2011.02.015

[9] M. Kanagasahapathy, H. Sasaki, S. Haldar, S. Yamasaki, and S. Nagata, Antibacterial activities of marine epibiotic bacteria isolated from brown algae of Japan, Annals of Microbiology, 56 (2006) 167–173.

DOI: 10.1007/bf03175000

[10] J. Park, J. Hong, H. Chul, S. Geun, S. Kim, J. Yoon, and Y. Jin, Bioresource Technology Use of Gelidium amansii as a promising resource for bioethanol : A practical approach for continuous dilute-acid hydrolysis and fermentation, Bioresource Technology, (2012).

DOI: 10.1016/j.biortech.2011.12.065

[11] G. L. Miller, Use of Dinitrosalicyclic Acid Reagent for Determination of Reducing Sugar, Analytical Chemistry, 31 (1959) 426–428.

DOI: 10.1021/ac60147a030

[12] Z. Anwar, M. Gulfraz, M. J. Asad, M. Imran, Z. Akram, S. Mehmood, A. Rehman, P. Anwar, and A. Sadiq, Bioethanol productions from rice polish by optimization of dilute acid pretreatment and enzymatic hydrolysis, African Journal of Biotechnology, 11 (2012).

DOI: 10.5897/ajb11.1145

[13] J. G. Black, Microbiology, seventh ed. John Wiley & SOns, Inc., Asia, (2008).

[14] Y. Khambhaty, K. Mody, M. R. Gandhi, S. Thampy, P. Maiti, H. Brahmbhatt, K. Eswaran, and P. K. Ghosh, Kappaphycus alvarezii as a source of bioethanol, Bioresource Technology, 103 (2012) 180–185.

DOI: 10.1016/j.biortech.2011.10.015