Control Study on Contamination Rate of Tissue Culture of Paris Polyphylla

Abstract:

Taking young and tender bud of Paris polyphylla as explants, this paper studies the influence of different disinfection time, different pretreatment of low temperature, penicillin pretreatment in different time, and different bacteriostat treatment on the contamination rate of the tissue culture of Paris polyphylla. The experimental results show that: the disinfection effects of 30ss alcohol disinfection combining with 10mins sterilizing by mercuric chloride are the best; 4°C low temperature pretreatment for 360min is helpful to decrease the contamination rate; soaking in penicillin for 10min is effective for decreasing the contamination; respectively adding 3000mg/L sodium propionate and 20mg/L sodium benzoate in nutrient medium can effectively reduce the contamination rate.

• Periodical: Advanced Materials Research (Volumes 926-930)
• Pages: 975-980
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.926-930.975
• Online since: May 2014
• Authors: Hua Yang, Fan Liu, Wen An, Meng Liang Tian*
• Keywords: Callus, Contamination Rate, Disinfection, Paris Polyphylla, Sterilization

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DOI: 10.1007/978-94-015-8951-2_19

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