Investigation of Solvent-Dependent Catalytic Behavior of a Hydrophobic Guest Artificial Glutathione Peroxidase

Abstract:

The investigation of the catalytic behavior of a hydrophobic guest artificial glutathione peroxidase (GPx) (ADA-Te-ADA) was carried out employing H₂O₂ and 3-carboxyl-4-nitrobenzenethiolas (TNB) as substrates. The relation between the catalytic rate of ADA-Te-ADA and the property of solvent used in the determination of catalytic activity was revealed. Typically, the co-solvents including ethanol, DMSO, DMF and CH₃CN were employed in the determination of catalytic rates. It indicated that ADA-Te-ADA exhibited the typical solvent-dependent catalytic behavior. Especially, the higher catalytic rate was observed when polar protic solvent (ethanol) was used compared with other co-solvents. It suggested that polar protic solvent was the appropriate co-solvent for the assay of catalytic activity of hydrophobic artificial GPx. Additionally, the strong polarity of polar aprotic solvent plays an important role in the enhancement of GPx catalytic activity. This study bodes well for the understanding of the catalytic behavior of hydrophobic guest artificial GPx.

• Periodical: Advanced Materials Research (Volume 940)
• Pages: 20-23
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.940.20
• Online since: June 2014
• Authors: Yan Zhen Yin*, Shu Fei Jiao, Xiong Gan, Zhong Feng Shi, Xiao Xi Hu, Yun Wang
• Keywords: Artificial Enzymes, Biomimetics, Catalytic Behavior, Glutathione Peroxidase

References

[1] L. Flohé, G. Loschen, W.A. Günzler and E. Eichele, Hoppe-Seyler's Z Physiol. Chem. 353, (1972) 987.

DOI: 10.1515/bchm2.1972.353.1.987

[2] N. Ezirmik, S. Taysi, R. Celik, G. Celik, H. A. Alici, H. Turhan, M. Cesur and D. Keskin. Asian. J. Chem., 20, (2008), (1950).

[3] A. Cebi, E. Diraman and Z. Eren, Asian. J. Chem., 21, (2009), 1359.

[4] Y. Yin, L. Wang, H. Jin, C. Lv, S. Yu, X. Huang, Q. Luo, J. Xu and J. Liu, Soft Matter., 7, (2011). 2521.

[5] Y. Yin, Z. Dong, Q. Luo and J. Liu, Prog. Polym. Sci., 37, (2012), 1476.

[6] H. Sies and H. Masumoto, Advances in pharmacology., 38, (1996), 229.

[7] B.K. Sarma and G. Mugesh, J. Am. Chem. Soc., 127, (2005), 11477.

[8] X. Zhang, H. Xu, Z. Dong, Y. Wang, J. Liu and J. Shen, J. Am. Chem. Soc., 126, (2004), 10556.

[9] S. Yu, X. Huang, L. Miao, J. Zhu, Y. Yin, Q. Luo, J. Xu, J. Shen and J. Liu, Bioorg. Chem., 38, (2010), 159.

[10] Y. Z. Yin, C. Lang, X. X. Hu, Z. F. Shi, Y. Wang, S. F. Jiao, C. X. Cai and J. Q. Liu, Russ. J. Bioorg. Chem., 40, (2014), 178.

[11] Z.P. Wu and D. Hilvert, J. Am. Chem. Soc., 112, (1990), 5647.