Improving the Yields of Carotene from Red Yeast by Using Ultra High Pressure and its Mutagenic Mechanism

Abstract:

The R. glutinis NR98 of high-producing β-carotene was treated by ultra high pressure (UHP) of 100~500 MPa for 10~30 min. The survival curve of NR98 treated for 10 min was saddle, which shows that the effect of UHP on this strain maybe was the cumulation of many biology effects. The rate of β-carotene production from mutant G39 (obtained at 300 MPa for 10 min) was increased by 59.87% compared with the initial strain NR98, and its genetic quality was proved to be stable by experiments. The result of restriction fragment length polymorphism analysis suggested that mutant strain G39 was likely to change in nucleic acid level.

• Periodical: Advanced Materials Research (Volumes 941-944)
• Pages: 1016-1020
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.941-944.1016
• Online since: June 2014
• Authors: Sui Lou Wang*, Hai Xiang Wang, Zhi Ping Yang
• Keywords: Mutation, Rhodotorula glutinis, Ultra High Pressure

References

[1] P. B. Bhosale, R. V. Gadre: Production of β-carotene by a mutant of Rhodotorula glutinis. Applied and Environment Microbiology. 55(44). (2001), pp.423-427.

DOI: 10.1007/s002530000570

[2] E. D. Simova, G. I. Frengova, D. M. Beshkova: Synthesis of carotenoids by Rhodotorula rubra GED8 co-cultured with yogurt starter cultures in whey ultra-filtrate. Journal of Industrial Microbiology and Biotechnology. 31(3). (2004), p.115–121.

DOI: 10.1007/s10295-004-0122-0

[3] A. Çelekli,  G. Dönmez. Effect of pH, light intensity, salt and nitrogen concentrations on growth and β-carotene accumulation by a new isolate of Dunaliella sp. World Journal of Microbiology and Biotechnology. 22 (2). (2006), pp.183-189.

DOI: 10.1007/s11274-005-9017-0

[4] P. B. Bhosale, R. V. Gadre. Manipulation of temperature and illumination conditions for enhanced β-carotene production by mutant32 of Rhodotorula glutinis. Letters in Applied Microbiology. 34 (5). (2002), pp.349-353.

DOI: 10.1046/j.1472-765x.2002.01095.x

[5] V. Perrier, E. Dubreucq, P. Galzy. Fatty acid and carotenoid composition of Rhodotorula strains. Archives of Microbiology. 164 (33). (1995), pp.173-179.

DOI: 10.1007/bf02529968

[6] S. L Wang, X. Z. Wu, L. H. Hao, J. S. Sun. Mutation effect of ultra high pressure on microbe. Acta Microbiologica Sinica (In Chinese). 45 (6). (2005), pp.970-973.

[7] L. Erijman, R. M. Clegg. Reversible stalling of transcription elongation complexes by high pressure. Biophysical Journal. 75 (1). (1998), pp.453-462.

DOI: 10.1016/s0006-3495(98)77533-2

[8] G. W. Niven, C. A. Miles, B. M. Mackey. The effects of hydrostatic pressure on ribosome conformation in Escherichia coli: an in vivo study using differential scanning calorimetry. Microbiology (UK). 145 (2). (1999), pp.419-425.

DOI: 10.1099/13500872-145-2-419

[9] M. G. Ganzle, R. F. Vogel. On-line fluorescence determination of pressure mediated outer membrane damage in Escherichia coli. System Applied Microbiology. 24 (4). (2001) , pp.477-485.

DOI: 10.1078/0723-2020-00069

[10] M. Ritz, J. L. Tholozan, M. Federighi, M. F Pilet. Physiological damages of Listeria monocytogenes treated by high hydrostatic pressure. International Journal of Food Microbiology. 79 (1). (2002), pp.47-53.

DOI: 10.1016/s0168-1605(02)00178-2

[11] S. L. Wang, X. Z. Wu, X. C. Duan, J. S. Sun. Mutagenic effects of ultra high pressure on strains producing laccase. Industrial Microbiology (In Chinese). 36 (2). (2006), pp.31-35.

[12] S. Basak, H. S. Ramaswamya, J. P. G. Piette. High pressure destruction kinetics of Leuconostoc mesenteroides and Saccharomyces cerevisiae in single strength and concentrated orange juice. Innovative Food Science & Emerging Technologies. 3 (3). (2002).

DOI: 10.1016/s1466-8564(02)00008-5

[13] L. Wu, Z. L. Yu. Radiobiological effects of a low-energy ion beam on wheat. Radiation and Environmental Biophysics (In Chinese). 40 (1). (2001), pp.53-57.

DOI: 10.1007/s004110000078

[14] E. Y. Wuytack, A. M. J. Diels, C. W. Michiels. Bacterial inactivation by high-pressure homogenisation and high hydrostatic pressure. International Journal of Food Microbiology. 77 (3). (2002), pp.205-212.

DOI: 10.1016/s0168-1605(02)00054-5

[15] H. Alpas, L. Alma,  F. Bozoglu. Inactivation of Alicyclobacillus acidoterrestris vegetative cells in model system, apple, orange and tomato juices by high hydrostatic pressure. World Journal of Microbiology and Biotechnology. 19 (6). (2003).

[16] D. H. Bartlett, C. Kato, K. Horikoshi. High pressure influences on gene and protein expression. Research in Microbiology. 146 (8). (1995), pp.697-706.

DOI: 10.1016/0923-2508(96)81066-7