Sensitive Determination of Total Microcystins in Water by Derivatization-Gaschromatography-Mass Spectrometry

Abstract:

The aim of the method development in this paper was to tackle the problems with the erythro-2-methyl-3-methoxy-4-phenylbutyric acid (MMPB) methodology: the rather laborious workflow and the loss of material during different steps of the method. In the optimised workflow, The detection limit of MMPB was 0.01 μg/L. Supposing the average of molecular weights of microcystins variants are 1000, the value of the detection limit is equivalent to 0.05 μg/L of microcystins. These results indicated that this method is the most sensitive method for determination of total microcystins, and it could be applied to the monitoring of microcystins in drinking water, reservoirs and freshwater lakes.

• Periodical: Advanced Materials Research (Volumes 955-959)
• Pages: 1321-1325
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.955-959.1321
• Online since: June 2014
• Authors: Hong Ping Pu, Qian Cai, Xue Jun Pan
• Keywords: Gc-Ms, Microcystins, MMPB, Silanization

References

[1] M. E. van Apeldoorn, H. P. van Egmond, G. J. Speijers, et al: Mol. Nutr. Food Res Vol. 51 (2007), p.7.

[2] F. Enzo and T. Emannula: Crit. Rev. Toxicol Vol. 38 (2008), p.97.

[3] L. A. Lawton and P. K. J. Robertson: Chem. Soc. Rev. Vol. 28 (1999), p.217.

[4] X. Y. Xu, R. P. Yu, L. P. Wang et al: Anal. Methods. Vol. 5 (2013), p.1799.

[5] Y. G. Zuo, , K. Zhang, Y. J. Lin: J. Chromatogr. A. Vol. 1148 (2007), p.211.

[6] K. Fang, X. J. Pan, B. Huang et al: Analytical Chemistry Vol. 38(2010), p.743 (In Chinese).

[7] B. Huang, X. J. Pan, X. Wan et al: Analytical Chemistry Vol. 39(2011), p.449 (In Chinese).