A Fluoroimmunoassay Method Using Quantum Dot Labels for Detection of Melamine

Abstract:

An indirect competitive immunoassay using quantum dots (QDs) as the fluorescent label has been developed for detection of melamine in milk products. The method exhibited a response for melamine with IC₅₀ value of 11.3 ng mL^-1 and a detection limit of 0.27 ng mL^-1. Milk and milk powder samples spiked with melamine have been analyzed, and the method provided recoveries ranging from 84.2% to 108.0%, and a CV of 12.8%. The implementation of QDs provides for at least 30 fold improvement of detection limit when compared with optically-based ELISA screening methods that have been reported. The analytical performance in combination with simplicity of sample and antibody preparation provides the basis for a practical method for melamine detection.

• Periodical: Advanced Materials Research (Volumes 955-959)
• Pages: 1484-1490
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.955-959.1484
• Online since: June 2014
• Authors: Chong Lin Luan*
• Keywords: Immunoassay, Melamine, Quantum Dot (QD)

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