An Inverted Observation Technique for Interactions Between Titanium Oxide and Cells on Commercially Pure Titanium

Akira Watazu; Kay Teraoka; Hirofumi Kido; Kae Okamatsu; Yoshiyuki Nagashima; M. Morita; Masanori Matsuura; Naobumi Saito

doi:10.4028/www.scientific.net/KEM.309-311.363

Paper Titles

Comparison of Antibacterial Effect of Three Bioactive Glasses
p.345

Effect of Ion Release on Antibacterial Activity of Melt-Derived and Sol-Gel-Derived Reactive Ceramics
p.349

Sintering Effects on Mechanical Properties of Hydroxyapatite-Titanium Dioxide (HA-TiO₂) Composites
p.355

Sintering Effect on Mechanical Properties of Composites of Bovine Derived Hydroxyapatite (BHA) with Titanium
p.359

An Inverted Observation Technique for Interactions Between Titanium Oxide and Cells on Commercially Pure Titanium
p.363

Titanium Oxide (TiO₂) Coatings Produced on Titanium by Oxygen-Plasma Immersion and Cell Behaviour on TiO₂
p.367

Biocompatibility and Corrosion Behavior of BaTiO₃/TiO₂ Double Layers by Electrochemical Synthesis
p.371

Effects of Electrical Wave Form on Pore Size of Micro-Arc Oxidized TiO₂ Film
p.375

Titanium Oxide Layer with Micro and Nano Tubes on Titanium Substrate
p.379

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An Inverted Observation Technique for Interactions Between Titanium Oxide and Cells on Commercially Pure Titanium

Abstract:

Commercially pure titanium thin films were uniformly formed on inner surfaces of tissue culture dishes by DC sputtering method. Then, the thickness of the film was about 30nm and the films were thin titanium oxide layer on commercially pure titanium. MC3T3-E1 cells were normally cultured on the dishes. Then, The films on the dishes were not broken and did not decompose. After 24 hours, observations of the sample from the direction of the cells' bottom surfaces adhered the titanium oxide on the commercially pure titanium film by an inverted optical microscope succeeded. Therefore, the new technique is useful for observations the interactions between titanium oxide and cells.