A single cell with a two-layer electrolyte consisting of an yttria stabilized zirconia (YSZ, Y2O3 8 mol%) layer and an Sm-doped ceria (SDC, Sm2O3 20mol%) interlayer has been fabricated on porous YSZ-NiO anode support. The layer of YSZ electrolyte was prepared by modified electrostatic powder coating method and the SDC interlayer by screen-printed method on the green YSZ layer. After co-firing at 1400°C for 5 h, the two-layer film with a dense YSZ film of about 15μm and porous SDC film of about 25μm was fabricated. The performances of as-fabricated single cell using La0.8Sr0.2FeO3 as cathode were tested using H2-3% H2O as fuel and air as oxidant at 800°C. Results indicated that the peak power density of a single cell with SDC interlayer reaches 469 mW/cm2 at 800°C, obviously higher than that of without SDC interlayer, which is about 300 mW/cm2 at 800°C.