Photo-Oxidative Stress on Model Membranes – Studies by Optical Methods

Marcela Elisabeta Bărbînţă Pătraşcu; Nicoleta Maria Badea; Laura Ţugulea; Aurelia Meghea

doi:10.4028/www.scientific.net/KEM.415.29

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A New Approach in Evaluation Surface Properties of Natural Temporary Teeth from High Polluted Area
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Natural Compounds with Antioxidant Properties
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Photo-Oxidative Stress on Model Membranes – Studies by Optical Methods
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Biopolymer Thin Films for Photonics Applications
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Evaluation of Scavenger Properties of some Flavonoidic Vegetal Extracts Obtained from Crataegus monogyna Jacq.
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Spectral Investigations Concerning the Degradation Degree of Romanian Historical Leather
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HomeKey Engineering MaterialsKey Engineering Materials Vol. 415Photo-Oxidative Stress on Model Membranes –...

Photo-Oxidative Stress on Model Membranes – Studies by Optical Methods

Abstract:

Photophysical studies on oxidative stress induced by illumination with ultraviolet (UV) light from artificial light sources, were performed on small unilamellar liposomes (SUVs) as models of biomembranes. Different liposoluble antioxidants were incorporated in the liposome bilayer during the lipid film preparation. The quercetin containing bilayers exhibited a high tolerance to photooxidative stress. The light-driven reactive oxygen species (ROS) effects on liposomal membranes were monitored by optical methods: UV-VIS absorption, fluorescence and chemiluminescence (CL), exploiting the spectral properties of chlorophyll a (Chla) embedded into some liposome bilayers (Chla/lipid molar ratio = 1/100). The interaction between Chla and the other liposome components resulted in changes in their absorption and emission fluorescence properties.

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Periodical:

Key Engineering Materials (Volume 415)

Pages:

29-32

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.415.29

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Online since:

September 2009

Authors:

Marcela Elisabeta Bărbînţă Pătraşcu, Nicoleta Maria Badea, Laura Ţugulea, Aurelia Meghea

Keywords:

Chlorophyll-a, Liposomes, Photo-Oxidative Stress, Quercetin, α-Tocopherol-Acetate, β-Carotene

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