For Libraries For Publication Open Access Downloads About Us Contact Us
Paper Titles
Beam Pointing Precision Control by Using a Liquid Crystal Optical Phased Array
p.344
Detection of Indoor Formaldehyde Concentration Using LaSrFeO3-Doped SnO2 Gas Sensor
p.349
Polymer Based Micro Sensors Arrays for Ph and Glucose Monitoring
p.354
Fiber Bragg Grating Demodulation System Based on Equi-Intensity Cantilever Beam
p.359
Auto Focusing Confocal Laser Induced Fluorescence Detection System
p.364
Study on Measuring System for Characteristics and Distribution of Skylight Polarization
p.369
A Preliminary Study of Micro Heat Conduction by Hot-Tip Tribological Probe Microscope
p.374
Information Fusion in Human Eye Aberration Measurement
p.379
A Method for Mass Estimation of Loose Parts in Nuclear Power Plant Based on Support Vector Machine
p.384

Auto Focusing Confocal Laser Induced Fluorescence Detection System

Article Preview

Abstract:

Laser induced fluorescence (LIF) detection is one of the main means of Capillary Electrophoresis (CE) chip detection, in which the confocal detecting device is commonly used for its higher sensitivity and signal-to-noise ratio (SNR). Based on confocal LIF detection principle, the confocal laser induced fluorescence detecting system, which could realize the auto focusing, and auto tracking was presented, and it contained the confocal optical system, the microprocessor control system and the computer process system. This device can acquire the fluorescence data by PMT or the chip images by CCD, and 3-dimensional electric moving stage could be controlled to accomplish the auto focusing and auto tracking by image process. The device could detect or observe the CE chip data in real time.

You might also be interested in these eBooks
Measurement Technology and Intelligent Instruments IX View Preview

Info:

Periodical:

Key Engineering Materials (Volume 437)

Pages:

364-368

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.437.364

Citation:

Cite this paper

Online since:

May 2010

Authors:

Xue Fei Wang, Wei Ping Yan, Hai Ming Bai, Wei Li

Keywords:

Auto Focusing, Auto Tracking, Capillary Electrophoresis, Confocal Laser Induced Fluorescence Detection

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

© 2010 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

References

[1] A. Manz, N. Graber, H.M. Widmer: Sens. Actuator B. Vol. 1 (1990), p.244.

Google Scholar

[2] Kurt Seiler, Zhonghui H. Fan: Anal. Chem. Vol. 66 (1994), p.3485.

Google Scholar

[3] Zhaolun Fang: Micro Fluidic Chip (Science Press, Beijing 2003).

Google Scholar

[4] Partik Vrabel, Petr Taborsky: J. Lumin. Vol. 118 (2006), p.283.

Google Scholar

[5] Katsumi Uchiyama, Hizuru Nakajima: Anal Bioanal Chem. Vol. 379 (2004), p.375.

Google Scholar

[6] Xihui Liang, Weineng Ou: Laser & Optoelectronics Progress. Vol. 1 (2008), p.65.

Google Scholar

[7] Jiang Guifeng, Said Attiya: Bisosensors & Bioelectronics. Vol. 14 (2000), p.861.

Google Scholar

[8] Bin He, Tianyu Ma, Yunjian Wang: Visual C++ Digital Image Processing (Posts & Telecom Press, Beijing 2002).

Google Scholar

[9] Qiushi Yi, Hongmin Zhang: Computer Engineering and Applications. Vol. 43 (2007), p.119.

Google Scholar

[10] A. Santos, C. Ortis De Solorzano: Journal of Microscopy. Vol. 188 (1997), p.264.

Google Scholar

[11] Sun Jie, Yuan Yuehui, Wang Chuanyong: Acta Optica Sinica. Vol. 27 (2007), p.63.

Google Scholar

[12] Chunsen Zhang, Weihong Cui: Computer Engineering and Applications. Vol. 26 (2004), p.89.

Google Scholar

© 2025 Trans Tech Publications Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies. For open access content, terms of the Creative Commons licensing CC-BY are applied.
Scientific.Net is a registered trademark of Trans Tech Publications Ltd.