Portable-Surface Plasmon Resonance Biosensor Immunoassays for the Human Serum Albumin Detection

Abstract:

The screening of urine human serum albumin (HSA) is significant for the early diagnosis and intervention of the nephropathia. This study developed sensitive HSA detection assay with a self-made portable-SPR biosensor based on the reflection light intensity interrogation. Anti-HSA monoclonal antibody was immobilized onto the gold sensing area and a directive assay for HSA was developed. A detection range of 1–100μg/mL HSA was found. The described label-free, real-time immunoassay for the quantification of HSA in urine is a rapid, sensitive, alternative method available for the clinic detection.

• Periodical: Key Engineering Materials (Volumes 562-565)
• Pages: 408-411
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.562-565.408
• Online since: July 2013
• Authors: Hui Li, Lu Lu Zhang, Hao Yuan Cai, Xing Chen, Jian Hai Sun, Ya Peng Chao, Da Fu Cui
• Keywords: Detection, Human Serum Albumin, Surface Plasmon Resonance Biosensor

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