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Silk Fibroin/Nano-CaP Bilayered Scaffolds for Osteochondral Tissue Engineering

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Abstract:

In this study, bilayered silk and silk/nanoCaP scaffolds were developed for osteochondral tissue engineering. Aqueous silk solution (16 wt.%) was used for preparation of the cartilage-like layer and, for generation of the silk/nanoCaP suspension and the bottom layer (CaP/Silk: 16 wt.%). The scaffolds were formed by using salt-leaching/lyophilization approach. The scanning electron microscopy revealed that the both layers presented porous structure and integrated well. Micro-computed tomography images confirmed that the CaP phase was only retained in the silk/nanoCaP layer. The hydration degree and mechanical properties of the bilayered scaffold were comparable to the ones of each single layer. The apatite crystal formation was limited to the silk/nanoCaP layer, when soaking the scaffold in a simulated body fluid solution, which is a must for the application of the developed scaffolds in OC tissue engineering.

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Periodical:

Key Engineering Materials (Volume 587)

Pages:

245-248

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.587.245

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Online since:

November 2013

Authors:

Le Ping Yan, Joaquim Miguel Oliveira, Ana L. Oliveira, Rui L. Reis

Keywords:

Bilayered Scaffold, Nanocalcium Phosphate, Osteochondral Tissue Engineering, Silk Fibroin

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© 2014 Trans Tech Publications Ltd. All Rights Reserved

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