Characterization of Hydroxyapatite from Chicken Bone via Precipitation

Abstract:

In this study, chicken bone was used as starting material for hydroxyapatite (HAp) synthesis. Chicken bone was calcined in electric furnace at 1000°C for 5 hours in order to obtain calcium oxide. Calcium oxide was reacted with aquadest to form calcium dioxide which was used as calcium precursor. Calcium dioxide was reacted with phosphoric acid which was used as phosphorus precursor. This reaction was conducted at pH 10 and reaction temperature was 40°C. The obtained results of this reaction were calcined at 500°C and 900°C for 2 hours and 6 hours. Then it was characterized by using FTIR, XRD and SEM. The increasing of temperature and time of calcination lead to low carbonate content and good morphology. Different result occurs in particle size which increased with increasing temperature and time of calcination. HAp that synthesized from this study had low purity. Best result was obtained in calcination at temperature 900°C and in 6 hours long where crystal size and crystallinity degree from this sample were 63.021 nm and 87.30% respectively.

• Periodical: Key Engineering Materials (Volume 744)
• Pages: 485-489
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.744.485
• Online since: July 2017
• Authors: Harahap Hamidah*, Iriany Iriany, Zuqni Meldha
• Keywords: Biogenic Source, Biomaterial, Calcination, Carbonate, Precipitation

References

[1] P. Szulc, M. L. Bouxsein, Overview of Osteoporosis: Epidemiology and Clinical Management, Vertebral Fracture Initiative Resource Document Part 1. (2011).

[2] S. Kannan, F. Goetz-Neunhoeffer, J. Neubauer, J. M. F. Ferreiraw, Ionic Substitutions in Biphasic HAp and B-Tricalcium Phosphate Mixtures: Structural Analysis by Rietveld Refinement, J. Am. Ceram. Soc. 91 (2008) 1–12.

DOI: 10.1111/j.1551-2916.2007.02117.x

[3] E. M. M. Ewaisa, Y. M. Z. Ahmeda, and S. M. El-Sheikhba, Refractory and Ceramic Materials, Journal of Asian Ceramic Societies. 3 (2015) 108–115.

[4] C. Y. W. Heng, X. Zheng, M. Liu, D. Xu, H. Huang, F. Deng, J. Hui, X. Zhang, Fabrication of Luminescent HAp Nanorods through Surface-Initiated RAFT Polymerization: Characterization, Biological Imaging and Drug Delivery Applications, Appl. Surf. Sci. 386 (2016).

DOI: 10.1016/j.apsusc.2016.05.157

[5] S. Rujitanapanicha, P. Kumpapanb, P. Wanjanoic, Synthesis of HAp from Oyster Shell via Precipitation, Energ. Proc. 56 (2014) 112–117.

[6] M. Supova, Substituted HAps for Biomedical Applications: A Review, Ceram. Int. 41 (2015) 9203–9231.

[7] A. Sobczak-Kupiec, Z. Wzorek, The influence of Calcination Parameters on Free Calcium Oxide Content in Natural HAp, Ceram. Int. 38 (2012) 641–647.

DOI: 10.1016/j.ceramint.2011.06.065

[8] K. Haberko, M. M. Bucko, W. Mozgawa, A. Pyda, J. Brzezinska-Miecznik, J. Carpentier, Behaviour of Bone Origin HAp at Elevated Temperatures and in O2 and CO2 atmospheres, Ceram. Int. 35 (2009) 2537–2540.

DOI: 10.1016/j.ceramint.2009.02.008

[9] A. Sobczak, Z. Kowalski, Z. Wzorek, Preparation of HAp from animal bones, Acta of Bioeng. Biomech. 11(4) (2009) 23–28.

[10] Suryadi. Synthesis and Characterization of Biomaterials HAp with Wet Chemical Deposition Process. Thesis, Univ. of Indonesia, Jakarta, Indonesia, (2011).

[11] W. Khoo, F. M. Nor, H. Ardhyananta, D. Kurniawan, Preparation of Natural HAp from Bovine Femur Bones Using Calcination at Various Temperatures, Proc. Manuf. 2 (2015) 196–201.

DOI: 10.1016/j.promfg.2015.07.034

[12] Y. X. Ooi, M. Hamdi, S. Ramesh, Properties of HAp Produced By Annealing of Bovine Bone, Ceram. Int. 33 (2007) 1171–1177.

DOI: 10.1016/j.ceramint.2006.04.001

[13] Z. N. Al-Sokanee, A. A. H. Toabi, M. J. Al-Assadi, E. A. S. Alassadi, The Drug Release Study of Ceftriaxone from Porous HAp Scaffolds, AAPS Pharm. Sci. Tech. 10(3) (2009) 772-779.

DOI: 10.1208/s12249-009-9265-7

[14] M. G. Ruiz, J. M. Hernandez, L. Banos, J. N Montes, M. E. R. Gracia, Characterization of Calcium Carbonate, Calcium Oxide and Calcium Hydroxide as Starting Point to the Improvement of Lime for Their Use in Construction, J. Mater. Civil Eng. 100 (2009).

DOI: 10.1061/(asce)0899-1561(2009)21:11(694)

[15] H. Elhendawi, R. M. Felfel, M. Bothaina, M. A. El-Hady, F. M. Reicha, Effect of Synthesis Temperature on the Crystallization and Growth of In Situ Prepared NanoHAp in Chitosan Matrix, Biomater. 2014 (2014) 897468.

DOI: 10.1155/2014/897468

[16] A. Achton, Calcium Phosphates – Advances in Research and Application, Edition: Scholarly Brief, (2013), p.175.