Capabilities of Four Microorganisms for Bioremediation of Lead Contaminated Soil

Abstract:

This work studied the potentials of indigenous Micrococcus sp., Bacillus sp., Pseudomonas sp., and Escherichia coli (E. coli) for bioremediation of lead contaminated soils collected from Amita forest in Ebonyi State of Nigeria.The organisms isolated from the soils were conditioned with the predetermined optimum factors in inoculated soil samples. The samples were tested for residual lead concentration at times 8, 16, 24, 32, 40, 48, and 56 days with Atomic Absorption Spectrophotometer.The performances of the organisms were in the decreasing order of Micrococcus sp., Bacillus sp., Pseudomonas sp., and E. coli. Micrococcus sp. and Bacillus sp. performed earlier at time 16 days as against Pseudomonas sp., and E. coli at 24 days. The maximum efficiencies were discovered at time 56 days as 76.68%, 72.24%, 70.11% and 55.47% for Micrococcus sp., Bacillus sp., Pseudomonas sp., and E. coli respectively with respective residual concentrations of 31.55 mg/kg, 37.55 mg/kg, 40.44 mg/kg and 60.24 mg/kg at the respective efficiencies.The rates of removals were in the decreasing order of -0.0524d^-1 for Pseudomonas sp., -0.0714 d^-1 for Bacillus sp., -0.0743d^-1 Micrococcus sp., and 0.113 d^-1 E. coli. The fitted models showed diffusion as the rate-limiting step for removals by Pseudomonas sp., Bacillus sp., and Micrococcus sp.; while chemisorption was the rate-limiting step for removal by E. coli. This information will be helpful to researchers and decision makers for the remediation of lead contaminated soils.

• Periodical: Key Engineering Materials (Volume 838)
• Pages: 125-134
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.838.125
• Online since: April 2020
• Authors: Edobor Kingsley Osagie*
• Keywords: Bioremediation, Contaminated Soils, Efficiency, Lead, Microorganisms

References

[1] C. Ainza, J. Trevors, M. Saier, Environmental Mercury rising, Wat. Air. Soil. Poll., 205 (2010) 47- 48.

DOI: 10.1007/s11270-007-9523-9

[2] A.V. Ajaykumar, A.D. Nalf, N. Hilal, Study of Various Parameters in the Biosorption of Heavy Metals on Sludge, Worl. App. Sci. J., 5 (2009) 32-40.

[3] M. Athar, S.B. Vohora, Heavy Metals and Environmental, New Age International (P) Limited Publishers, 4835/24, Ansari Road, Daryaganji, New Delhi 2006, 110002.

[4] E. Atikpo, M.O. Ezugwu, Preferential Removal of Lead, Cadmium and Zinc from Contaminated Agricultural soil by Bacillus subtilis, J. Nig. Assoc. Math. Phys. 41 (2017) 339-346.

[5] E. Atikpo, A. Michael, Performance Evaluation of Six Microorganisms Ultilized for the Treatment of Lead Contaminated Agricultural Soil, J. Appl. Sci. Envi.. Manag. 22(7) (2018) 1105-1109.

DOI: 10.4314/jasem.v22i7.18

[6] E. Atikpo, Spatial Distribution and Attenuation of Heavy Metals Pollution in AmaonyeIshiagu Forest Soils, A PhD Thesis Submitted to the Department of Civil Engineering, University of Benin, Benin City, Nigeria, (2016).

[7] E. Atikpo, B.U. Anyata, Variation of Factors for Metals Removal: A Guide to Factors Selection for Bioremediation of Heavy Metals Polluted Soils in Amaonye-Ishiagu of Ebonyi State in Nigeria. J. Civ. Envi. Systems Eng. 13 (2015) 172-181.

[8] R.K. Badawy, A.M.A. El-Gawad, H.E. Osman, (2013). Health Risks Assessment of Heavy Metals and Microbial Contamination in Water, Soil and Agricultural Foodstuff from Waste Water Irrigation at Sahl El-Hessania area, Egypt, J. App. Sci. Res., 9 (4) (2013) 3091-3107.

[9] M.A.O. Badmus, T.O.K. Audu, B.U. Anyata, Removal of Lead Ion from Industrial Wastewaters by Activated Carbon Prepared from Periwinkle Shells (Typanotonusfuscatus), Turk. J. Envi. Engi. Sci. 31 (2007) 251-263.

DOI: 10.1007/s11814-007-5049-5

[10] E.J. Baron, L.R. Peterson, S.M. Finegold, Bailey and Scotts Diagnostic Microbiology, 9th Edition. Mosby, Baltimore, (1994).

[11] A. Begum, M. Ramaiah, O. Harikrishna, K. Irfanulla, K. Veena, Analysis of Heavy Metal Concentrations in Soil and Litchen from Various Localities of Hosur Road, J. Chem. 6(1) (2009) 13-22.

DOI: 10.1155/2009/943695

[12] H. Chen, A. Wang, Kinetic and Isothermal Studies of Lead Ion Adsorption onto Palygorskite Clay, J. Coll. Int. Sci., 307 (2007) 309-316.

DOI: 10.1016/j.jcis.2006.10.054

[13] M. Chessebrough, Disrict Laboratory Pactice in Troppical Countries, Part 2, Cambridge low Price Edition, Cambridge University Press, London, (2000).

[14] S.H. Chien, W.R. Clayton, Application of Elovich Equation to the Kinetics of Phosphate Release and Sorption on Soil, J. Soil Sci. 44 (1980) 265-268.

DOI: 10.2136/sssaj1980.03615995004400020013x

[15] S.T. Cowan, Cowan and Steels Manual for the Identification of Medical Bacteria, Cambridge University Press, London, (1993).

[16] S.T. Cowan, R. Steel, Manual for the Identification of Medical Bacteria, Cambridge University Press, London, (1990).

[17] S.M. Daboor, Application of Bacterial Biomass as A Potential Heavy Metal Bio-Removal Agent, Afr. J. Micr. Res., 8(22) (2014) 2229-2237.

DOI: 10.5897/ajmr2014.6837

[18] H.N. Ezeh, E. Chukwu, Small Scale Mining and Heavy Metals Pollution of Agricultural Soils: The Case of Ishiagu Mining District, South Eastern Nigeria, J. Geo. Min. Res. 3(4) (2011) 87-104.

[19] R.V. Galiulin, V.N. Bashkin, R.A. Galiulina, R. Kucharski, (2002). Airborne Soil Contamination by Heavy Metals in Russia and Poland, and its Remediation, Land Cont. Recl. 10(3) (2002) 179-187.

DOI: 10.2462/09670513.607

[20] Y.S. Ho, D.A. John Wase, C.F. Forster, Study of the Sorption of Divalent Metal Ions onto Peat, Adsor. Sci. Tech. 18 (2000) 639-650.

DOI: 10.1260/0263617001493693

[21] J.C. Holt, The Shorter Bergeys Manual of Determinative Bacteriology, Eight Edition, Williams and Willkins Company, Baltimore, (1994).

[22] C. Lin, H. Lin, Remediation of soil contaminated with the heavy metal (Cd2+), J. Hazar. Mat. 122(1-2) (2005) 7-15.

[23] G. Mckay, V.J. Poots, Kinrtics and Diffussion Processes in Colour Removal from Effluent Using Wood as an Adsorbent, J. Che. Tech. Biotech., 30 (1980) 279-292.

DOI: 10.1002/jctb.503300134

[24] S. Murthy, G. Bali, S.K. Sarangi (2012).Biosorption of Lead by Bacillus Cereus Isolated from Industrial Effluents, Brit. Biot. J. 2(2) (2012) 73-84.

[25] S. Nanda, J. Abraham, Remediation of Heavy Metal Contaminated Soil, Afr. J. Biotech. 12(2) (2013) 3099-3109.

[26] V.O. Nwaugo, R.A. Onyeagba, E.I. Akubugwo, S. Ugbogu, (2008). Soil Bacterial Flora and Enzymatic Activities in Zinc and Lead Contaminated Soil, Biok. 20(2) (2008) 77-84.

[27] G.O. Onyedika, G.U. Nwosu, Lead, Zinc and Cadmium in Root Crops from Mineralized Galena-Sphalerite Mining Areas and Environment, Pak. J. Nutr., 7(3) (2008) 418-420.

DOI: 10.3923/pjn.2008.418.420

[28] H.I. Owamah, Biosorptive removal of Pb (II) and Cu (II) from wastewater using activated carbon from cassava peels, J. Mat. Cycl. Wast. Manag. 16(2) (2014) 347-358.

DOI: 10.1007/s10163-013-0192-z

[29] A.K. Pendias, H. Pendias, Elements of Group VIII. In: Trace Elements in Soils and Plants. Boca Raton: CRC Press, (1992) 271-276.

DOI: 10.1017/s0014479711000743

[30] D.P. Samarth, C.J. Chandekar, Bhadekar, Biosorption of heavy Metals from Aqueous Solution using Bacillus Licheniformis, Int. J. Pure Appl. Sci. Tech., 10(2) (2012) 12-19.

[31] K.Vaxevanidou, N. Papassiopi, I. Paspaliaris, (2008). Removal of Heavy Metals ans Arsenic from Contaminated Soil using Bioremediation and Chelant Extraction Techniques, Chemos. 70 (2008) 1329-1337.

DOI: 10.1016/j.chemosphere.2007.10.025

[32] M. Vidali, Bioremediaton, an Overview, Pure Apl. Chem., 73 (2001) 1163-1172.

[33] P.M. White, D.C. Wolf, G.J. Thomas, C.M. Reynolds, Phytoremediation of Alkylated Polycyclic Aromatic Hydrocarbon in an Oil-Contaminated Soil, Wat. Air Soil Poll., 169 (2006) 207-220.

DOI: 10.1007/s11270-006-2194-0

[34] World Health Organization, Health Guidelines for the Use of Waste Water in Agriculture and Aquaculture, Report of a Scientific Group Meeting, Technical Report Series, No. 778, World Health Organisation, Geneva, (2001).