Conjugation of Antibody on Gold Nanoparticles for Biosensors Application

Sarocha Ta-Aithuak; Naruenard Loedsapchinda; Nongluck Houngkamhang

doi:10.4028/www.scientific.net/KEM.853.92

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HomeKey Engineering MaterialsKey Engineering Materials Vol. 853Conjugation of Antibody on Gold Nanoparticles for...

Conjugation of Antibody on Gold Nanoparticles for Biosensors Application

Abstract:

Regarding to the unique optical property and biocompatible, gold nanoparticles have been widely used to functionalize with antibodies probe for testing specificity with their antigen targets. The antibody immobilized onto gold nanoparticles which synthesized by different methods were studied. Gold nanoparticles synthesized by citrate reduction method and by using poly (ethylene) glycol (PEG) coated gold nanoparticles were immobilized with IgM antibody by physical adsorption. Gold nanoparticles before and after functionalize with antibodies were characterized with fourier-transform infrared spectroscopy (FTIR) for functional group and UV-Vis spectroscopy for absorption wavelength. Finally, a specificity test was conducted using spot of anti-IgM antibody onto nitrocellulose membrane to confirm the bioactivity of antibodies attached to gold nanoparticles.

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Periodical:

Key Engineering Materials (Volume 853)

Pages:

92-96

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.853.92

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Online since:

July 2020

Authors:

Sarocha Ta-Aithuak, Naruenard Loedsapchinda, Nongluck Houngkamhang*

Keywords:

Antibody, Conjugation, Gold Nanoparticles (GNPs), Immobilization

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