The Influence of Zinc Ions in Insulin Fibrillation by Heat at Acid Solution Revealed Using Small Angle X-Ray Scattering

Abstract:

The fibrillation mechanism of insulin in acid solution has been studied by small angle X-ray scattering (SAXS). It was observed that insulin monomer unfolded in both conditions. Furthermore, in zinc free solution, insulin tend to aggregate on heating start in the first 5 min. The fibrillation through aggregation process continues until 30 min on heating. The similar phenomenon occurs in the presence of zinc ions. The SAXS data suggest that the presence of zinc ions prevent the long cylindrical fibril at the beginning of heating. However, after 20 min heating, the large cylindrical fibril of insulin formed in both conditions.

• Periodical: Key Engineering Materials (Volume 884)
• Pages: 327-334
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.884.327
• Online since: May 2021
• Authors: Arum Patriati*, Nadi Suparno, Siriwat Soontaranon, Edy Giri Rachman Putra
• Keywords: Acid, Aggregation, Heat, Insulin, SAXS, Zinc

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