Extraction and Characterization of Keratin from Chicken and Swiftlet Feather

Abstract:

Keratin is a durable and fibrous protein of hair, nails, horns, hoofs, feathers and the epithelial cells in the outermost layers of the skin. Keratin in animals mainly presents in vertebrates such as mammals, birds and reptiles including chicken and swiftlet. This study aims to characterize keratin extracted from chicken and swiftlet feathers. The extraction of the keratin performed using dimethyl sufoxide (DMSO) at high temperature. The extracted keratin from both samples were used for the characterization process using Bradford protein assay, CHNS analysis and Fourier-transform infrared (FTIR) spectroscopy. This study showed that keratin extract of swiftlet feather showed higher protein concentration (0.813 mg/mL) than keratin extract of chicken feather (0.646 mg/ml). The highest composition for keratin extract is hydrogen which are 4.97% for keratin extract from swiftlet feathers and 3.12% for keratin extract from chicken feathers. FTIR analysis exhibited that carboxyl groups and amino groups are presence in both keratin samples however, the protein value is higher in swiftlet feathers compared to chicken feathers. This study's outcome is significant in discovering keratin extract from swiftlet feathers containing high protein content due to the breakdown of disulfide bonds. Furthermore, this research is the first report on keratin characterization from swiftlet feathers that would be useful for high value future keratin study.

• Periodical: Materials Science Forum (Volume 1025)
• Pages: 157-162
• DOI: https://doi.org/10.4028/www.scientific.net/MSF.1025.157
• Online since: March 2021
• Authors: Sharifah Zafierah Syed Badrulzaman, Aimi Wahidah Aminan, Aizi Nor Mazila Ramli*, Rohaida Che Man, Nur Izyan Wan Azelee
• Keywords: Chicken Feather, Extraction, Keratin, Protein, Swiftlet Feather

References

[1] Utomo, B. and DjalalRosyidi, L. Cleaning Method by Keratinase Enzyme for Improving Quality Edible Bird Nest, Journal of Life Science Biomedicine. 4 (2014) 416-420.

[2] Kumawat, T. K., Sharma, A., Sharma, V. and Chandra S. Keratin. New York, (2018).

[3] Mothé, M. G., Viana L.M. and Mothé C. G. Thermal property study of keratin from industrial residue by extraction, processing and application, Journal of Thermal Analysis and Calorimetry. 131 (2018) 417–462.

DOI: 10.1007/s10973-017-6845-8

[4] Pourjavaheria, F., Pour, S. O., Jones, O. A. H., Smooker P. M., Brkljača R., Sherkat, F., Blanch E. W., Gupta A. and Shanks R. A. Extraction of keratin from waste chicken feathers using sodium sulfide and l-cysteine, Process Biochemistry Journal. 82 (2019) 205-214.

DOI: 10.1016/j.procbio.2019.04.010

[5] Rahayu, S., Suhartono, M. T., Suryapratama, W., & Bata, M. Keratinolytic Enzymes for Cleaning Edible Bird's Nest. Biosciences, Biotechnology Research Asia. 14 (2017) 989–996.

DOI: 10.13005/bbra/2533

[6] Shavandi, A., Bekhit, A. E. B., Carne, A. and Bekhit, A. Evaluation of keratin extraction from wool by chemical methods for bio-polymer application, Journal of Bioactive and Compatible Polymers. 32 (2016) 1–15.

DOI: 10.1177/0883911516662069

[7] Gupta, A., Kamarudin, N. B., Kee, C. Y. G. and Yunus, R. B. M. Extraction of Keratin Protein from Chicken Feather, Journal of Chemistry and Chemical Engineering. 6 (2012) 732-737.

[8] Wang, Y. X. and Cao, X. J. Extracting keratin from chicken feathers by using a hydrophobic ionic liquid, Process Biochemistry. 47 (2012) 896–899.

DOI: 10.1016/j.procbio.2012.02.013

[9] Khumalo, M., Sithole B. and Tesfaye T. Valorisation of waste chicken feathers: Optimisation of keratin extraction from waste chicken feathers by sodium bisulphite, sodium dodecyl sulphate and urea, Journal of Environmental Management. 262 (2020) 1-7.

DOI: 10.1016/j.jenvman.2020.110329

[10] Alahyaribeik, S and Ullah A. Methods of keratin extraction from poultry feathers and their effects on antioxidant activity of extracted keratin, International Journal of Biological Macromolecules. 1 (2020) 1-31.

DOI: 10.1016/j.ijbiomac.2020.01.144

[11] Tjernberg, A., Markova, N. T., Griffiths, W. J. and Hallén D. DMSO-Related Effects in Protein Characterization, Journal of Biomolecular Screening. 11 (2006) 131-137.

DOI: 10.1177/1087057105284218

[12] Sinkiewicz, I., Śliwińska, A., Staroszczyk, H. and Kołodziejska, I. Alternative Methods of Preparation of Soluble Keratin from Chicken Feathers, Waste Biomass Valor. 8 (2017) 1043–1048.

DOI: 10.1007/s12649-016-9678-y

[13] Kang, N., Hails, C. J. and Sigurdsson, J. B. Nest construction and egg-laying in Edible-nest Swiftlets Aerodramus spp. and the implications for harvesting, lbis. 133 (2008) 170-177.

DOI: 10.1111/j.1474-919x.1991.tb04828.x

[14] Cooper, G. M. The Cell: A Molecular Approach (2nd ed). Sunderland, England, (2000).

[15] Fortunati, E., Aluigi, A., Armentano, I., Morena, F., Emiliani, C., Martino, S., Santulli, C., Torre, L., Kenny, J.M. and Puglia D. Keratins extracted from Merino wool and Brown Alpaca fibres: Thermal, mechanical and biological properties of PLLA based biocomposites, Materials Science and Engineering C. 47 (2015) 394–406.

DOI: 10.1016/j.msec.2014.11.007

[16] Shanmugasundaram, O. L., Ahmed, K. S. Z., Sujatha, K. Ponnmurugan, P., Srivastava, A., Ramesh, R., Sukumar, R. and Elanithi K. Fabrication and characterization of chicken feather keratin/polysaccharides blended polymer coated nonwoven dressing materials for wound healing applications, Materials Science & Engineering. 92 (2017) 1-7.

DOI: 10.1016/j.msec.2018.06.020

[17] Sudhir, M. R. Isolation and Identification of Feather Degrading Bacteria from Poultry Farm Soil and Characterization of Enzyme. Thesis. Narsee Monjee Institute of Management Studies, Mumbai. Shodhganga. Web (2007).

[18] Chai, T. T., Xiao, J., Dass, S. M., Teoh, J. Y., Ee, K. Y., Ng, W. J., & Wong, F. C. Identification of antioxidant peptides derived from tropical jackfruit seed and investigation of the stability profiles, Food Chemistry. 340 (2021) 1-6.

DOI: 10.1016/j.foodchem.2020.127876

[19] Price, V. H. and Menefee, E. On The Effect of Dimethyl Sulfoxide on Hair Keratin, The Journal of Inynstigative Dermatology. 49 (1967) 297-301.

DOI: 10.1038/jid.1967.139

[20] Torreggiani, A., Foggia, M. D., Manco I., Maio A. D., Markarian S. A. and Bonora S. Effect of sulfoxides on the thermal denaturation of hen lysozyme: A calorimetric and Raman study, Journal of Molecular Structure. 891 (2008) 115–122.

DOI: 10.1016/j.molstruc.2008.03.007

[21] Mokrejš, P., Huťťa, M., Pavlačková, J. and Egner P. Preparation of Keratin Hydrolysate from Chicken Feathers and Its Application in Cosmetics, Journal of Visualized Experiments. 129 (2017) 1-9.

DOI: 10.3791/56254-v

[22] Wadhwani, T., Desai, K., Patel, D., Lawani, D., Bahaley, P., Joshi, P. and Kothari, V. Effect of various solvents on bacterial growth in context of determining MIC of various antimicrobials, The Internet Journal of Microbiology. 7 (2008) 1-6.

DOI: 10.5580/b43

[23] Haddar, A., Hmidet, N., Ghorbel-Bellaaj, O., Fakhfakh-Zouari, N., Sellami-Kamoun, A. and Nasri M. Alkaline Proteases Produced by Bacillus licheniformis RP1 Grown on Shrimp Wastes: Application in Chitin Extraction, Chicken Feather degradation and as a Dehairing Agent, Biotechnology and Bioprocess Engineering. 16 (2011) 669-678.

DOI: 10.1007/s12257-010-0410-7

[24] Garett, R. H. and Grisham C. M. Biochemistry (5th ed). United State, (2013).

[25] Sadelhoff, J. H. J. V., Mastorakou, D., Weenen, H., Stahl,B., Garssen, J. and Hartog. A. Short Communication: Differences in Levels of Free Amino Acids and Total Protein in Human Foremilk and Hindmilk, Nutrients. 10 (2018) 1-8.

DOI: 10.3390/nu10121828

[26] Voets, I. K., Cruz, W. A., Moitzi, C., Lindner, P., Arêas, E. P. G. and Schurtenberger P. DMSO-Induced Denaturation of Hen Egg White Lysozyme, Journal of Physical Chemistry B. 114 (2010) 11875–11883.

DOI: 10.1021/jp103515b

[27] Alashwal, B. Y., Gupta A. and Husain M. S. B. Characterization of dehydrated keratin protein extracted from chicken feather, Materials Science and Engineering. 702 (2019) 1-8.

DOI: 10.1088/1757-899x/702/1/012033

[28] Eslahi, N., Dadashian, F. and Nejad, N. H. An Investigation on Keratin Extraction from Wool and Feather Waste by Enzymatic Hydrolysis, Preparative Biochemistry and Biotechnology. 43 (2013) 624-648.

DOI: 10.1080/10826068.2013.763826