Green Synthesis of TiO₂ Nanoparticles Using Piper Longum Leaf Extract: Morphological, Optical and Antibacterial Characterization

Abstract:

In this study, Titanium dioxide nanoparticles (TiO₂ NPs) were synthesized using Piper longum leaf extract as both a capping and reducing agent through a green synthesis approach. The synthesized TiO₂ NPs were thoroughly characterized using multiple techniques: X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), UV-Diffuse reflectance spectroscopy (UV-DRS), field emission scanning electron microscopy (FESEM), High-Resolution Transmission Electron Microscopy (HR-TEM), Brunauer-Emmett-Teller (BET) analysis, and X-ray photoelectron spectroscopy (XPS). XRD analysis confirmed the anatase phase of TiO₂ with an average crystallite size of 57 nm. UV-DRS revealed a band gap of 3.39 eV, indicative of TiO₂ nanoparticle formation. FTIR spectroscopy identified biomolecules such as flavonoids, phenol compounds, and tannins around the nanoparticles. SEM images showed a variety of shapes, including hexagons, pentagons, triangles, and tetragons. EDX analysis confirmed the presence of titanium, carbon, and oxygen, verifying the purity of the TiO₂ nanoparticles. BET analysis determined a specific surface area of 59 m²/g. The antibacterial efficacy of the TiO₂ nanoparticles was assessed using the agar well diffusion method, demonstrating significant antibacterial activity against Pseudomonas aeruginosa, Streptococcus mutans, Staphylococcus aureus, and Klebsiella pneumoniae. Additionally, the photocatalytic degradation of methylene blue dye by the TiO₂ NPs achieved an efficiency of 96%.

• Periodical: Materials Science Forum (Volume 1143)
• Pages: 73-88
• DOI: https://doi.org/10.4028/p-8cpLZy
• Online since: December 2024
• Authors: A. Mary George Shemona*, S. Kanagaprabha, H. Johnson Jeyakumar, S. Arul Vathana
• Keywords: Antibacterial Activity, Morphological Characterize, Optical Studies, Piper Longum, TiO₂ Nanoparticles

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