Implementation of Penicillium sp. as Raw Material for Synthesizing Metal Nanoparticles for Antibiosis

Abstract:

Silver Nanoparticles (Ag-NPs) have been known to have inhibitory and bactericidal effects. Resistance to antimicrobial agents by pathogenic bacteria has emerged in recent years and is a major health problem. The ability of some microorganisms such as bacteria and fungi to control the synthesis of metallic Nanoparticles should be employed in the search for new materials. The present work is the study of biosynthesis of silver Nanoparticles synthesized from different Penicillium spp. The Penicillium species were identified by employing microscopic methods and laboratory manuals. The Nanoparticles synthesis was done by extracellular and intracellular methods. The silver Nanoparticles formed were characterized by Uv-vis spectrophotometer followed by TEM analysis. The maximum absorbance 410nm confirmed the formation of silver Nanoparticles and TEM revealed the particle size in the range of 30-40nm, which after confirmation was checked for its antibacterial activity against selected microbial pathogens like Bacillus cereus, Proteus vulgaris, Staphylococcus aureus and Staphylococcus epidermidis. The Nanoparticles synthesized by Penicillium species showed a well anti microbial activity that was further compared with different antibiotics to check the Nanoparticles activity which can be substituted in place of high dose antibiotics.

• Periodical: Materials Science Forum (Volume 760)
• Pages: 33-38
• DOI: https://doi.org/10.4028/www.scientific.net/MSF.760.33
• Online since: July 2013
• Authors: B.K. Nayak, M. Amin Bhat, Anima Nanda
• Keywords: Extra Synthesis, Intracellular Synthesis, Silver Nanoparticle, TEM, UV-Visible Spectroscopy

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