Online Glucose Measurement in DMEM for In Vitro Cell Culture

Abstract:

In-vitro cell culture offers the ability to grow individual cells and monitor their behaviour in a controlled environment over a certain time. During culture, different parameters have to be controlled to ensure a vital growth of cells. The most important parameters are temperature, pH-value, oxygen and carbon dioxide levels as well as the glucose concentration. All of these parameters influence the growth ability of the cell culture and should be monitored online. The online monitoring of glucose was achieved with a novel GOD based sensor which enabled a real-time measurement of glucose during cell culture. A first-time characterisation of the sensor was carried out in the culture medium DMEM. In addition, material properties of the culture system were investigated. At first to establish a baseline measurement, the sensor was placed in different glucose concentrations dissolved in Water-KCl mixture at 25 °C inside a batch chamber under constant stirring. Afterwards, the temperature was increased to 37 °C to simulate a cell culture environment. In addition, the system was transferred into a flow-through reaction chamber. The highest response signal and the most stable signal was achieved at 37 °C with DMEM during flow-through measurement. Based on these measurements online glucose monitoring in cell culture was possible to determine the glucose consumption for each cell which is important for future human-on-a-chip devices to prevent diabetic metabolisms.

• Periodical: Materials Science Forum (Volume 941)
• Pages: 2501-2506
• DOI: https://doi.org/10.4028/www.scientific.net/MSF.941.2501
• Online since: December 2018
• Authors: Nelson Matuschek*, Thilo Liebscher, Loredana Santo, Andreas H. Foitzik
• Keywords: Biosensor, Cell Culture, Cells, Micro-Reactor, Online Glucose Monitoring

References

[1] S. Princz, U. Wenzel, S. Schwarz, N. Rotter, and M. Hessling, New bioreactor vessel for tissue engineering of human nasal septal chondrocytes,, Curr. Dir. Biomed. Eng., vol. 2, no. 1, Jan. (2016).

DOI: 10.1515/cdbme-2016-0071

[2] Stefan Scholz, Erika Sela, Ludek Blaha, and Thomas Braunbeck, Malyka Galay-Burgos, Mauricio García-Franco, Joaquin Guinea, Nils Klüver, Kristin Schirmer, Katrin Tanneberger, Marysia Tobor-Kapłon, Hilda Witters, Scott Belanger, Emilio Benfenati, Stuart Creton, Mark T.D. Cronin, Rik I.L. Eggen, Michelle Embry, Drew Ekman, Anne Gourmelon, Marlies Halder, Barry Hardy, Thomas Hartung, Bruno Hubesch, Dirk Jungmann, Mark A. Lampi, Lucy Lee, Marc Léonard, Eberhard Küster, Adam Lillicrap, Till Luckenbach, Albertinka J. Murk, José M. Navas, Willie Peijnenburg, Guillermo Repetto, Edward Salinas, Gerrit Schüürmann, Horst Spielmann, Knut Erik Tollefsen, Susanne Walter-Rohde, Graham Whale, James R. Wheeler, Matthew J. Winter, A European perspective on alternatives to animal testing for environmental hazard identification and risk assessment,, Regul. Toxicol. Pharmacol., vol. 67, no. 3, p.506.

[3] BUNDESMINISTERIUM FÜR ERNÄHRUNG UND LANDWIRTSCHAFT, BMEL - Übersicht: BMEL informiert über Tierschutz - Verwendung von Versuchstieren im Jahr 2016,, 2016.

[4] B. Alberts, A. Johnson, J. Lewis, M. Raff, K. Roberts, and P. Walter, Molecular Biology of the Cell, 4th ed. Garland Science, (2002).

[5] C.-F. Mandenius, Ed., Bioreactors: design, operation and novel applications. Weinheim: Wiley-VCH Verlag GmbH & Co. KGaA, (2016).

[6] J. W. Haycock, 3D cell culture: a review of current approaches and techniques,, Methods Mol. Biol. Clifton NJ, vol. 695, p.1–15, (2011).

[7] M. M. Ward Muscatello, L. E. Stunja, and S. A. Asher, Polymerized Crystalline Colloidal Array Sensing of High Glucose Concentrations,, Anal. Chem., vol. 81, no. 12, p.4978–4986, Jun. (2009).

DOI: 10.1021/ac900006x

[8] K. Tian, M. Prestgard, and A. Tiwari, A review of recent advances in nonenzymatic glucose sensors,, Mater. Sci. Eng. C, vol. 41, p.100–118, Aug. (2014).

DOI: 10.1016/j.msec.2014.04.013

[9] C. Chen et al., Recent advances in electrochemical glucose biosensors: a review,, RSC Adv., vol. 3, no. 14, p.4473, (2013).