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Real-Time Cytotoxicity Assay of Water Extracts Hibiscus rosa-sinensis

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Abstract:

A real-time impedance-based electrochemistry assay was used for assessing the cytotoxicity effect of water extract Hibiscus Rosa-Sinensis on Vero cell line. In this study, H. rosa-sinensis was extracted by using the water extraction with three different concentration which were 200, 100 and 50 µg/mL. The phytochemical compounds in the extract were analyzed by using the Gas Chromatography-Mass Spectrometry (GC-MS). The GC-MS result showed that the extract contains bioactive compounds such as n-hexadecanoic acid, pentadecanoic acid, phenol, 2,4-bis(1,1-dimethylethyl) and octadecanoic acid that have bioactive properties such as cytotoxic and antioxidant. The cytotoxic effect of the extract on normal cell line was assessed by using Real-Time Cell Analysis (RTCA) instrument. The result showed that there was no significant cytotoxicity effect against Vero cell lines. This result showed that the real-time impedance-based assay can be utilized to monitor changes of the cells and to determine the inhibitory concentration of the extract.

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Periodical:

Materials Science Forum (Volume 981)

Pages:

265-270

DOI:

https://doi.org/10.4028/www.scientific.net/MSF.981.265

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Online since:

March 2020

Authors:

Nur Husnina Iffah Bakar, Thong Chuan Lee, Ab. Rahim Mohd-Hairul, Raha Ahmad Raus, Dayang Fredalina Basri, Noor Suhana Adzahar*

Keywords:

Cytotoxicity, Hibiscus, RTCA, Vero Cell

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© 2020 Trans Tech Publications Ltd. All Rights Reserved

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