Influence of Sieving Polymer Concentration and Applied Voltage on Separation Accuracy of the Capillary Gel Electrophoresis for Small DNA Fragments

Tomoka Nakazumi; Yusuke Hara

doi:10.4028/www.scientific.net/MSF.990.101

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HomeMaterials Science ForumMaterials Science Forum Vol. 990Influence of Sieving Polymer Concentration and...

Influence of Sieving Polymer Concentration and Applied Voltage on Separation Accuracy of the Capillary Gel Electrophoresis for Small DNA Fragments

Abstract:

In this study, measurement conditions of Capillary gel electrophoresis (CGE) were optimized in order to increase the separation accuracy for small DNA fragments. We adopted a 20-bp DNA Ladder including 13 small double-stranded fragments (20, 40, 60, 80, 100, 120, 140, 160, 180, 200, 300, 400, and 500-bp). The applied voltage and sieving polymer concentration were assessed because these two factors significantly affect the separation accuracy of DNA fragments. As a result, we succeeded in optimizing CGE separation conditions for small DNA fragments with a sufficient degree of accuracy.

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Periodical:

Materials Science Forum (Volume 990)

Pages:

101-105

DOI:

https://doi.org/10.4028/www.scientific.net/MSF.990.101

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Online since:

May 2020

Authors:

Tomoka Nakazumi, Yusuke Hara*

Keywords:

Capillary Gel Electrophoresis (CGE), DNA, Electroosmotic Flow (EOF), Point-of-Care Testing (POCT)

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* - Corresponding Author

References

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