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Experimental 2.1 Material and reagents Low molecular protein markers were purchased from Fermentas (Lithuania) or Bio-Rad (Richmond, CA, USA).
L-Arginine, guanidine hydrochloride (GuHCl), isopropylthiogalactoside (IPTG) and phenylmethylsulfonylfluoride (PMSF) were purchased from Beijing Solarbio Science & Technology Co., Ltd (Beijing, China).
Reference [1] Frederic G, Hayden M.D, Antiviral Resisitance in influenza Virus-Implications for Management and Pandemic Response, The New England Journal of Medicine. 354(2006)785-788
[5] Earl Justice Smith, Amantadine-induced Psychosis in a Yong Healthy Patient, The American Journal of Psychiatry. 165 (2008)1613
[7] Meijuan Xu, Wenzhang Ju, Xiaoyan Xia, et al, Determination of rimantadine in rat plasma by liquidchromatography/electrrospray mass spectrometry and its application in a pharmacrokinetic study, Journal of Chromatography B. 864 (2008)123-128

Tribon XML file includes the geometry, material, thickness and other physical attribute information of the model.
Journal of Marine Science and Technology. 2008, 13(2): 154-163
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The Information View collects the knowledge about objects (material and information) as they are used and produced in the course of the enterprise operations [7].
Feng : Studies of integrated electronic information system information delivery probability evaluation models, in Journal of China Academy of Electronics and Information Technology, Vol. 2 (2007), p. 271-318 [3].
Dai: Research on evaluation methods about the index of time of integrated electronic information system, in Journal of China Academy of Electronics and Information Technology, Vol. 2 (2007), p. 234-8 [4].
Wang: Fuzzy Integrated Evaluation of E-commerce information System, in International Symposium on Computer Science and Computational Technology, Vol. 2 (2008), p. 513-516  [5].
Songhori: An integrated Delphi/VAHP/DEA framework for evaluation of information technology/information system (IT/IS), in International Journal of Advanced Manufacturing Technology, Vol. 45 (Dec 2009), p.1233-1251 [6].

Material was 40Cr and forging billet.
Acknowledgment The work presented in this paper has been supported by A Key Project of Higher Education Natural Science Foundation of Anhui Province of China under Grant No.
The International Journal of Advanced Manufacturing Technology,2011,53( 1- 4) : 339- 351
International Journal of Production Research, 2008,46(4): 929-947
International Journal of Production Research [J]. 2008, 46（4）: 929-947

Unsteady seepage theory As for the homogeneous isotropic material, the differential equation for one-dimensional unsteady seepage is presented as follows[6]: (1) Where is the permeability coefficient; is the unit storage amount, namely the released water in saturated soil of unit volume by soil compression and water expansion when unit water head drops.
Acknowledgements This work was supported by the National Natural Science Foundation of China (Grant Nos. 51139001, 51179066, 51079046), the Special Fund of State Key Laboratory of China(Grant Nos. 2010585212).
Chinese Journal of Geotechnical Engineering, 2011,33(11):1807-1811,(in Chinese)
Journal of Hydroelectric Engineering, 2010,29(4):200-206,(in Chinese)
Journal of Tian jin University, 2007,40(7): 779-785,(in Chinese)

Nichols, Volume of Fluid Method for Dynamic of Free Boundaries, Journal of Computational Physics, 39 (1975) 201-225
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Youngs, Time-Dependent Multi-Material Flow With Large Fluid Distortion, Academic Press, New York, 1982. [7] J.
Zema, A Continuum Method for Modeling Surface Tension, Journal Computational Physics, 100 (1992) 335-354
Oriolo, Surface Tension of Lithium Bromide Solution With Heat-Transfer Addidives, Journal Chemical Engineering, 36 (1991) 96-98

There are lots of evidences in the literatures [1-9] indicating that grain boundary plays important roles in the mechanical behavior of polycrystalline materials.
This work will help us to understand deeply the damage and fracture mechanisms and their processing of the polycrystalline materials. 2.
For polycrystalline materials, lots of grain boundaries exist in material, so it is difficult to predict the stress distribution near the grain boundary.
In order to discuss the mechanism of material, it is concluded that homogenous assumption for polycrystalline materials is not suitable to study the meso mechanisms of the damage and fracture and their detail processing.
It is concluded that homogenous assumption for polycrystalline materials is not suitable to study the meso mechanisms of the damage and fracture and their detail processing.

For example, the event “delayed arrival of construction material” can cause a delay in execution.
Events can affect activities, material or work resources, lags, and calendars in project execution process.
Risk assessment and allocation in the UAE construction industry, International Journal of Project Management, 26 (2008) 431-438
Mulholland, J.C., Risk Assessment in Construction Schedules, Journal of Construction Engineering and Management, Vol. 125, No. 1, (1999) 8-15
An Uncertainty Importance Measure of Activities in PERT Networks, International Journal of Production Research, 1964 (12): p. 460-470

Freudenthal suggests that the Weibull distribution might be appropriate when the material parameter (here the fatigue life) is subject to poor quality control and the lognormal distribution might be appropriate when the material parameter is subject to good quality control [9].
Journal of applied physics.
International Journal of Fracture.Vol.14(1978), p.327
Extreme values and related models with applications in engineering and science.
Journal of Structural Division, ASCE.

Today, materials with high thermal hardness, high tensile strength, and sufficient thermal conductivity such as powder metallurgy (PM) high-speed steels are more frequently used.
These developments pose challenges to fine machining operations and require further investigation of the process and the cutting materials [2].
DeVor: Mechanistic model for the reaming process with emphasis on process faults, International Journal of Machine Tools and Manufacture, 46 (2006), 836-846
DeVor: The effects of process faults and misalignment on the cutting force system and hole quality in reaming, International Journal of Machine Tools and Manufacture, 46 (2006), 1281-1290
In Journal of Manufacturing Science and Engineering 123 (2001), 387