Microorganism Precipitation in Enhancing Concrete Properties

Article Preview

Abstract:

Microorganism is an unique living element and has the ability to precipitate minerals through the process of biomineralisation. The precipitation process occured naturally and most of the precipitated products are very important compound composed of such as carbon, nitrogen, oxygen, sulphur, phosphorus and silica. So far, concrete incorporated with microorganism that able to precipitate calcium carbonate (calcite) was reported. However, little information on silica precipitation and its effect on concrete properties has been revealed. In this present study, the concrete specimens were incorporated with Bacillus subtilis silica adsorbed in their cell wall. Concrete specimens with five different concentration of Bacillus subtilis cell which are 104, 105, 106 and 107 cell/ml and control (without Bacillus subtilis) were cast. The experimental investigation aims to prove that the silica precipitated by this microorganism can enhance the concrete properties namely its compressive strength and resistance to carbonation. The microstructure of the concrete contained Bacillus subtilis was also examined. It appears that the inclusion of Bacillus subtilis into the concrete enhanced the compressive strength. The concentration of 106 cell/ml was found to be the optimum concentration to give most enhanced effect to the compressive strength. However the effect of including Bacillus subtilis to the resistance to carbonation of the concrete specimen is found to be insignificant.

You might also be interested in these eBooks

Info:

Periodical:

Pages:

1157-1165

Citation:

Online since:

September 2011

Export:

Price:

Permissions CCC:

Permissions PLS:

Сopyright:

© 2011 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

[1] Urrutia, M.M. & Beveridge, T.J. Formation of fine grained metal and silicate precipitates on a bacterial surface (Bacillus subtilis). Chemical Geology, 116, pp.261-280, (1994)

DOI: 10.1016/0009-2541(94)90018-3

Google Scholar

[2] Lowenstam, H.A. and Weiner, S.W. On Biomineralization. Oxford University Press, New York,1989.

Google Scholar

[3] Lam, S.S., Fortin, D., Davis, B.S. and Beveridge, T.J. Mineralization of Bacterial Surfaces, Chemical Geology, 132, pp.171-181, 1996.

DOI: 10.1016/s0009-2541(96)00053-8

Google Scholar

[4] Buczynski, C. & Chafetz, H.S., Habit of Bacterially induce precipitates of calcium carbonate and the influence of medium viscosity on mineralogy, J. Sediment. Petrol., 61, 1991, pp.226-233

DOI: 10.1306/d42676db-2b26-11d7-8648000102c1865d

Google Scholar

[5] Douglas, S. & Beveridge, T.J. Mineral formation by bacteria in natural microbial communities. FEMS Microbial. Ecol., 26, 1998, pp.79-88

DOI: 10.1111/j.1574-6941.1998.tb00494.x

Google Scholar

[6] Ehrlich, H.L. Microbes as geologic agent: Their role in mineral formation, Geomicrobiol. J., 16, p.1999, 135-153

Google Scholar

[7] Inagaki, F., Motomura, Y. And Ogata, S. Microbial Silica Deposition in Geothermal Hot Waters. Appl Microbiol Biotechnol 60, pp.605-611, 2003.

DOI: 10.1007/s00253-002-1100-y

Google Scholar

[8] Lowenstam, H.A. Minerals Formed by Organisms. Science, 211, pp.1126-1131, 1981.

DOI: 10.1126/science.7008198

Google Scholar

[9] Biswas, M., Majundar, S., Chowdury, T., Chattopadhyay, B., Mandal, S., Halder, U. & Yamasaki, S.Bioremediase a unique protein from a novel bacterium BKH1, ushering a new hope in concrete technology. Enzyme and Microbial Technology, 46, pp.581-587, 2010.

DOI: 10.1016/j.enzmictec.2010.03.005

Google Scholar

[10] Bang, S.S., Galinat, J.K. &Ramakrishnan, V. Calcite precipitation induced by polyurethane-immobilzed Bacillus pasteurii. Enzyme and Microbial Technology, 28, pp.404-409, 2001.

DOI: 10.1016/s0141-0229(00)00348-3

Google Scholar

[11] Dick, J., Windt, W., Graef, B., Saveyn, H., Meeren, P., De Belie, N. &Verstraete, W. Biodeposition of a Calcium Carbonate Layer on Degraded Limestone by Bacillus species.Biodegradation, 17 (4), pp.357-367, 2006.

DOI: 10.1007/s10532-005-9006-x

Google Scholar

[12] Fischer, S.S., Galinat, J.K. & Bang, S.S. Microbiological precipitation of CaCO3.Soil Biology and Biochemistry, 31, pp.1563-1571, 1999.

DOI: 10.1016/s0038-0717(99)00082-6

Google Scholar

[13] Santosh, K., Ramachandran, S.K., Ramakrishnan, V. & Bang, S.S. Remediation of Concrete Using Microorganisms, American Concrete Institute Journal, 98, pp.3-9, 2001.

Google Scholar

[14] Ramakrishnan, V., Panchalan, R.K. & Bang, S.S. Improvement of Concrete Durability by Bacterial Mineral Precipitation, Proceedings of 11th International Conference on Fracture, pp.20-25 March, Turin Italy, 2005.

Google Scholar

[15] Ghosh, S., Biswas, M., Chattopadhyay, B.D. &Mandal, S. Microbial activity on the microstructure of bacteria modified mortar. Cement & Concrete Composites, 31,pp.93-98, 2009.

DOI: 10.1016/j.cemconcomp.2009.01.001

Google Scholar

[16] Muynck, W.D., Cox, K., Belie, N.D. &Verstraete, W. (2008a). Bacterial carbonate precipitation as alternative surface treatment for concrete.Construction and Building Materials, 22, pp.875-885, 2008a.

DOI: 10.1016/j.conbuildmat.2006.12.011

Google Scholar

[17] Muynck, W.D., Cox, K., Belie, N.D. &Verstraete, W.Bacterial carbonate precipitation improves the durability of cementitious materials. Cement and Concrete Research, 38, pp.1005-1014,2008b

DOI: 10.1016/j.cemconres.2008.03.005

Google Scholar

[18] Tittelboom, K.V., Belie, N.D., Muynck, W.D. &Verstraete, W. Use of bacteria to repair cracks in concrete, Cement and Concrete Research, 40,p.157 – 166, 2010.

DOI: 10.1016/j.cemconres.2009.08.025

Google Scholar

[19] Inagaki, F., Yokoyama, T., Doi, K., Izawa, E. & Ogata, S. (1998) Biodeposition of Amorphous Silica by an Extremely Thermophilic Bacterium, Thermus spp. Biosci. Biotechnol.Biochem., 62(6), pp.1271-1272, (1998)

DOI: 10.1271/bbb.62.1271

Google Scholar

[20] Iwai, S., Doi, K., Fujino, Y., Nakazono, T., Fukuda, K., Motomura, Y. & Ogata, S. Silica Deposition and Phenotypic Changes to Thermus thermophilus Cultivated in the Presence of Supresaturated Silica. The ISME Journal, 4, pp.809-816, (2010)

DOI: 10.1038/ismej.2010.12

Google Scholar

[21] Mera, M.U. & Beveridge, T.J. Mechanism of Silicate Binding to the Bacterial Cell Wall in Bacillus subtilis.Journal of Bacteriology ,7 (175), pp.1936-1945, 1993.

DOI: 10.1128/jb.175.7.1936-1945.1993

Google Scholar

[22] Beveridge, T.J. & Murray R.G.E. Sites of metals deposition in the cell wall of Bacillus subtilis. J.Bacteriol, 141,pp.876-887, (1980)

DOI: 10.1128/jb.141.2.876-887.1980

Google Scholar

[23] Doyle, R.J., Matthews, T.H. & Streips, U.N. Chemical basis of metals ion by the Bacillus subtilis cell wall. J. Bacteriol,143, pp.471-480, 1980.

DOI: 10.1128/jb.143.1.471-480.1980

Google Scholar

[24] Urrutia, M.M. & Beveridge, T.J. Remobilization of Heavy Metals Retained as Oxyhydrixides or Silicates by Bacillus subtilis Cells. Applied And Environmental Microbiology, 59 (12) , pp.4323-4329, 1993.

DOI: 10.1128/aem.59.12.4323-4329.1993

Google Scholar

[25] Ghosh, P., Mandal, S., Chattopadhyay, B.D. & Pal, C. Use of microorganism to improve the strength of cement mortar. Cement and Concrete Research, 35, pp.1980-1983, 2005.

DOI: 10.1016/j.cemconres.2005.03.005

Google Scholar

[26] Barabesi, C., Galizzi, A., Mastromei, G., Rossi, M., Tamburini, E. & Perito, B. Bacillus subtilis Gene Cluster Involved in Calcium Carbonate Biomineralization, Journal of Bacteriology, 189(1), pp.228-235, (2007)

DOI: 10.1128/jb.01450-06

Google Scholar

[27] Fujita, Y., Redden, G.D., Ingram, J., Cortez, M.M., Ferris, F.G. & Smith, R. W. Strontium Incorporation into Calcite Generated by Bacterial Ureolysis, Geochimica et Cosmichimica Acta, 68 (15), 2004, 3261-3270

DOI: 10.1016/j.gca.2003.12.018

Google Scholar