MCP-1 Expression Associated with Inflammation and Biomaterials

Abstract:

The study of inflammation is important in understanding the reaction between biomaterials and the immune system. In the current study, human umbilical vein endothelial cells (ECV-304), cultured with PRMI-1640, were detached from cells cultured with the supernatant of macrophages which contained TNF-α and IL-1β because of stimulation by biomaterials. Cells were then put into contact with multiple biomaterials with different biocompatibilities. Levels of monocyte chemoattractant protein-1 (MCP-1) were measured. The results suggested that level of MCP-1 cultured with supernatant of macrophages was higher than PRMI-1640 in the same biomaterials. And, exposure to the positive control, Polytetrafluoroethylene, Poly-Lactic-co-Glycolic acid and American NPG alloy, resulted in high expression of MCP-1 (p< 0.001) following cytokine stimulation. MCP-1 was also significantly expressed in β-Tricalcium Phosphate and Calcium Phosphate Cement samples (p < 0.01). These data suggested that MCP-1 was activated by both cytokines and biomaterials.