Paper Titles
A Novel Polycaprolactone/Hydroxyapatite Scaffold for Bone Tissue Engineering
p.265
Evaluation of Semi-Interpenetrating Polymer Networks Composed of Chitosan and Poloxamer for Artificial Skin
p.269
Effects of Mechanical Stress on the In Vitro Degradation of Porous Composite Scaffold for Bone Tissue Engineering
p.273
Bone Regeneration Materials
p.277
Muscle-Derived Stem Cell/Polymer Hybrid Mixture for the Treatment of Urinary Incontinence
p.281
Investigation of Pore Size Effect on Cell Compatibility Using Pore Size Gradient Chitosan Scaffold
p.285
Fabrication and Characterization of Hydrophilized Polydioxanone Scaffolds for Tissue Engineering Applications
p.289
Characterization of Hydrophilized PCL Electrospun Sheet as an Efective Guided Bone Regeneration Membrane
p.293
Evaluation of RGD-Grafted Porous PLLA Scaffold for Chondrogenic Differentiation of Stem Cells
p.297

Muscle-Derived Stem Cell/Polymer Hybrid Mixture for the Treatment of Urinary Incontinence

Article Preview

Abstract:

In this study, muscle-derived stem cell (MDSC)/Pluronics/polycaprolactone (PCL) microparticle hybrid mixture was prepared as a potential injectable urethral bulking agent for the treatment of urinary incontinence. The MDSCs were isolated from the gastrocnemius muscles of SD rats by a modified preplate method and characterized through FACS analysis using various primary antibodies (CD34, Sca-1, CD45 and desmin). The hybrid mixture was prepared by the mixing of PCL microparticles (diameter, 100~200 μm) and MDSCs-containing thermo-sensitive Pluronic (F127/F68 mixture) solution (4.5/5.5, w/v). The hybrid mixture was easily injected through 18G needle into the body and stably remained in the applied site without initial volume decrease, owing to a well-packed structure of PCL particles exhibited in the hybrid mixture. It was observed that the MDSCs were stably grown in the hybrid mixture without severe inflammation and immune reaction. From the results, we recognized that the hybrid mixture can be a good candidate as an injectable bulking agent for the treatment of urinary incontinence, due to their good injectability, volume retention and biocompatibility.

You might also be interested in these eBooks
Advanced Biomaterials VII View Preview

Info:

Periodical:

Key Engineering Materials (Volumes 342-343)

Pages:

281-284

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.342-343.281

Citation:

Cite this paper

Online since:

July 2007

Authors:

Se Heang Oh, Jun Ho Kim, Ji Youl Lee, Sung Ho Ghil, Soon Hong Yuk, Jin Ho Lee

Keywords:

Bulking Agent, Muscle-Derived Stem Cell, Pluronic, Polycaprolactone, Urinary Incontinence

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

© 2007 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

References

[1] P Abrams, JG Blaivas, SL Stanton and JT Andersen: Scand. J. Urol. Nephrol. Suppl. 114 (1988), p.5.

Google Scholar

[2] J Blaivas and C Olsson: J. Urol. 157 (1997), p.818.

Google Scholar

[3] M Alcalay, QA Monga and SL Stanton: Br. J. Obstet. Gynaecol. 102 (1995), p.740.

Google Scholar

[4] S Madjar, K Jacoby, C Giberti, M Wald, S Halachmi, E Issaq, B Moskovitz, M Beyar and O Nativ: J. Urol. 165 (2001), p.72.

DOI: 10.1097/00005392-200101000-00018

Google Scholar

[5] Å Stenberg and G Läckgren: J. Urol. 154 (1995), p.800.

Google Scholar

[6] RR Dmochowski and RA Appell: Urology 56 (2000), p.32.

Google Scholar

[7] SH Oh, JY Lee, SH Ghil, SS Lee, SH Yuk and JH Lee: Biomaterials 27 (2006), p. (1936).

Google Scholar

[8] SH Oh, JK Kim, KS Song, SM Noh, SH Ghil, SH Yuk, and JH Lee: J. Biomed. Mater. Res., 72A (2005).

Google Scholar