Paper Titles
Wear of Resin Composites Polymerized by Conventional Halogen Light Curing and Light Emitting Diodes Curing Units
p.645
Study on Bio-Tribological Properties of Nitrogen Ion Implanted UHMWPE
p.649
3D Finite Element Analysis of Bone Stress around Distally Osteointegrated Implant for Artificial Limb Attachment
p.653
Preliminary Evaluation of a New Dental Implant Design with Finite Element Analysis
p.657
Refolding and Purification of rhBMP-2 Expressed as Inclusion Bodies in E.COLI with Hydroxyapatite Chromatography
p.661
Protection of Human Fibroblasts from Reactive Oxygen Species by Green Tea Polyphenolic Compounds
p.665
Periodic Structure of Blaze Grating in Abalone Shell
p.669
Research on the Parallel Arrangement of Aragonite Sheets in Clam's Shell
p.673
Polyurethane Foam with a Negative Poisson's Ratio for Diabetic Shoes
p.677

Refolding and Purification of rhBMP-2 Expressed as Inclusion Bodies in E.COLI with Hydroxyapatite Chromatography

Article Preview

Abstract:

The objective of this study was to develop an efficient method for the production of bioactive bone morphogenetic protein 2 (BMP-2). A recombinant plasmid encoding mature human BMP-2 was transferred and expressed at a high level in E.coli. Most of the aimed proteins existed in inclusion bodies. The non-active recombinant human BMP-2 (rhBMP-2) monomer in inclusion bodies was refolded and simultaneously purified using hydroxyapatite (HA) chromatography. After oxidization of the monomer, the rhBMP-2 dimmer showed biological activity by the induction of alkaline phosphate activity in C2C12 cells. The refolding yield was about 30% and the purity was about 90% just by one chromatography process.

You might also be interested in these eBooks
Advanced Biomaterials VI View Preview

Info:

Periodical:

Key Engineering Materials (Volumes 288-289)

Pages:

661-664

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.288-289.661

Citation:

Cite this paper

Online since:

June 2005

Authors:

Shaohua Yao, Ling Li Zhang, Steven Y. Cheng, Xing Dong Zhang

Keywords:

Hydroxyapatite Chromatography, Inclusion Bodies, Purification, Refolding , rhBMP-2

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

© 2005 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

References

[1] Rainer Ruppert, Elke Hoffmann, Walter Sebald. Eur. J. Biochem. 1996. 237: 295.

Google Scholar

[2] Wang EA. Rosen V. Cordes P etal. Proc. Natl. Acad. Sci. USA. 1988. 85: 9484.

Google Scholar

[3] Wang EA. Rosen V. D'Alessandro etal. Proc. Natl. Acad. Sci. USA. 1990. 87: 2220.

Google Scholar

[4] Xiong Shaohu, Yu Lei, Tan Haiyan etal. J. First Mil Med. Univ. 2002. 22(5): 413: 416.

Google Scholar

[5] Eliana De Bernordez Clark. Current Opinion in Biotechnology. 1998. 9: 157.

Google Scholar

[6] Zhenyu Gu. Zhiguo Su. Jan-Christer Janson. J. Chromatogr. A. 2001. 918: 311.

Google Scholar

[7] Ming Li. Guifeng Zhang. Zhiguo Su. J. chromatogr. A. 2002 959: 113-120.

Google Scholar

[8] Guo LA, Geng XD. Chinese Journal of Biotechnology. 2000. 16(6): 661.

Google Scholar

[9] G. Lemercier, N. Bakalara, X. Santarelli. J. Chromatogr. B. 2003. 786: 305.

Google Scholar

[10] Cerletti,N. Patent No. US. 6057430.

Google Scholar